Protein Info for EFB2_01603 in Escherichia fergusonii Becca

Annotation: UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 385 PF01041: DegT_DnrJ_EryC1" amino acids 18 to 379 (362 residues), 464.9 bits, see alignment E=4.3e-143 PF01053: Cys_Met_Meta_PP" amino acids 38 to 216 (179 residues), 35.1 bits, see alignment E=1.2e-12 PF00155: Aminotran_1_2" amino acids 41 to 169 (129 residues), 35.7 bits, see alignment E=1.2e-12 PF00266: Aminotran_5" amino acids 50 to 285 (236 residues), 24.9 bits, see alignment E=2e-09

Best Hits

Swiss-Prot: 100% identical to ARNB_ECO45: UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB) from Escherichia coli O45:K1 (strain S88 / ExPEC)

KEGG orthology group: K07806, UDP-4-amino-4-deoxy-L-arabinose-oxoglutarate aminotransferase [EC: 2.6.1.87] (inferred from 99% identity to ecg:E2348C_2397)

MetaCyc: 98% identical to UDP-4-amino-4-deoxy-L-arabinose aminotransferase (Escherichia coli K-12 substr. MG1655)
RXN0-1863 [EC: 2.6.1.87, 2.6.1.92]

Predicted SEED Role

"UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (EC 2.6.1.-)" in subsystem Lipid A-Ara4N pathway ( Polymyxin resistance ) (EC 2.6.1.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.6.1.-

Use Curated BLAST to search for 2.6.1.- or 2.6.1.87 or 2.6.1.92

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (385 amino acids)

>EFB2_01603 UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (Escherichia fergusonii Becca)
MTDGKKMSGFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQLTGNQHAIAV
SSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDVDRDTLMVTPEAI
EAAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVGTYYKGRHIGAKGTA
IFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDRHTWGRAPQAEVLTPGY
KYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPFQPLSLPAWPHVHAWHLFI
IRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYRERFPTLSLPNTEWNSERICS
LPLFPDMTTADADRVITALKQLAGQ