Protein Info for EFB2_01506 in Escherichia fergusonii Becca

Annotation: putative multidrug resistance protein EmrK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 387 signal peptide" amino acids 1 to 16 (16 residues), see Phobius details transmembrane" amino acids 17 to 34 (18 residues), see Phobius details TIGR00998: efflux pump membrane protein" amino acids 14 to 338 (325 residues), 483.8 bits, see alignment E=1.1e-149 PF00529: CusB_dom_1" amino acids 34 to 340 (307 residues), 61.6 bits, see alignment E=1.4e-20 PF13533: Biotin_lipoyl_2" amino acids 56 to 102 (47 residues), 35.8 bits, see alignment 1.4e-12 PF16576: HlyD_D23" amino acids 57 to 285 (229 residues), 49.4 bits, see alignment E=9.5e-17 PF13437: HlyD_3" amino acids 211 to 289 (79 residues), 47.4 bits, see alignment E=7.1e-16

Best Hits

Swiss-Prot: 98% identical to EMRK_ECOLI: Probable multidrug resistance protein EmrK (emrK) from Escherichia coli (strain K12)

KEGG orthology group: K07797, multidrug resistance protein K (inferred from 98% identity to eco:b2368)

MetaCyc: 98% identical to tripartite efflux pump membrane fusion protein EmrK (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-365

Predicted SEED Role

"Membrane fusion component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (387 amino acids)

>EFB2_01506 putative multidrug resistance protein EmrK (Escherichia fergusonii Becca)
MEQINSNKKHSDRRKYFALLAVVLFIAFSGAYAYWSMELKDMISTDDAYVTGNADPISAQ
VSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLANIVRQTNKLYLQDKQYSA
EVASARIQYQQSLEDYNRRVPLAKQGVISKEALEHTKDTLISSKAALNAAIQAYKANKAL
VMNTPLNRQPQVIEAADATKEAWLALKRTDIKSPVTGYIAQRSVQVGETVSPGQSLMAVV
PARQMWVNANFKETQLTDVRIGQSVNIISDLYGENVVFHGRVTGINMGTGNAFSLLPAQN
ATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSMTATIDTKNEDIAEMPDLASTVTSMPA
YTSKALVIDTSPIEKEISNIISHNGQL