Protein Info for EFB2_01481 in Escherichia fergusonii Becca

Annotation: putative cyclic di-GMP phosphodiesterase PdeA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 729 transmembrane" amino acids 12 to 31 (20 residues), see Phobius details amino acids 42 to 62 (21 residues), see Phobius details amino acids 82 to 104 (23 residues), see Phobius details amino acids 125 to 143 (19 residues), see Phobius details amino acids 163 to 186 (24 residues), see Phobius details amino acids 214 to 232 (19 residues), see Phobius details amino acids 238 to 255 (18 residues), see Phobius details amino acids 262 to 278 (17 residues), see Phobius details amino acids 290 to 312 (23 residues), see Phobius details PF05231: MASE1" amino acids 16 to 315 (300 residues), 218.5 bits, see alignment E=1.2e-68 PF00563: EAL" amino acids 493 to 724 (232 residues), 199.6 bits, see alignment E=5.1e-63

Best Hits

Swiss-Prot: 98% identical to PDEA_ECOLI: Probable cyclic di-GMP phosphodiesterase PdeA (pdeA) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to ecv:APECO1_4144)

Predicted SEED Role

"FIG00638309: hypothetical protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (729 amino acids)

>EFB2_01481 putative cyclic di-GMP phosphodiesterase PdeA (Escherichia fergusonii Becca)
MFVEHNLIKNIKIFTLAFTLTVVLIQLSRLISPLAVIHSNYIFLAWMPLCVMLSILFIFG
WRGVVPILCGMFCTNLWNLHLSFLQTAVMIGSQAFAVLCACAILRWQLGTRWRYGLTSRY
VWQRLFWLGLVAPIGIKCSMYLVGNFFDFPLKISTFFGDADAIFTVVDLLSLFTAVLIYN
MLFYYLTRMIVSPHFAQILWRRDIAPSLSKEKRAFTLSWLAALSVLLLLMCTPYENDFIA
GYLVPVFFIIFTLGVGKIRYPFLNLTWAVSTLCLLNYNQNFLQGVLTEYSLAFILAVLIS
FSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLLPNFRALEQAPEQEAGKSFCCLRIDNL
EFMSRHYGLIMRVHCIRSIYRTLLPLMQENEKLYQLPGSELLLVLSGPETEGRLQHMVNI
LNSRQIHWNNTGLDMGYGAAWGRFDGNQETLQPLLGQLSWLAEQSCAHHHVLALDSREEM
VSGQTTKQVLLLNTIRTALDQGDLLLYAQPIRNKEGEGYDEILARLKYDGGIMTPDKFLP
LIAQFNLSARFDLQVLESLLKWLATHPCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRY
YISPQAVILEITEEQAFSNAESSMYNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADII
KIDGVFVKDIVTNTLDAMIVRSITDLAKAKSLSVVAEFVETPQQQALLHKLGVQYLQGYL
IGRPQPLAD