Protein Info for EFB2_01324 in Escherichia fergusonii Becca

Annotation: Anti-sigma-E factor RseA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 216 transmembrane" amino acids 98 to 119 (22 residues), see Phobius details PF03872: RseA_N" amino acids 1 to 87 (87 residues), 130 bits, see alignment E=3.9e-42 PF03873: RseA_C" amino acids 132 to 186 (55 residues), 90 bits, see alignment E=9.2e-30

Best Hits

Swiss-Prot: 100% identical to RSEA_ECOLI: Anti-sigma-E factor RseA (rseA) from Escherichia coli (strain K12)

KEGG orthology group: K03597, sigma-E factor negative regulatory protein RseA (inferred from 100% identity to eco:b2572)

Predicted SEED Role

"Sigma factor RpoE negative regulatory protein RseA" in subsystem Transcription initiation, bacterial sigma factors

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (216 amino acids)

>EFB2_01324 Anti-sigma-E factor RseA (Escherichia fergusonii Becca)
MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMRGDTPEVLHFDISSRVM
AAIEEEPVRQPATLIPEAQPAPHQWQKIPFWQKVRPWAAQLTQMGVAACVSLAVIVGVQH
YNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANNGQQQQVQEQRRRINAMLQDY
ELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ