Protein Info for EFB2_00877 in Escherichia fergusonii Becca

Annotation: Antigen 43

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 948 transmembrane" amino acids 34 to 53 (20 residues), see Phobius details PF13018: ESPR" amino acids 6 to 50 (45 residues), 49.5 bits, see alignment 9.4e-17 TIGR04415: autotransporter passenger strand-loop-strand repeat" amino acids 128 to 162 (35 residues), 20.6 bits, see alignment (E = 1.8e-08) amino acids 164 to 191 (28 residues), 20.8 bits, see alignment (E = 1.5e-08) amino acids 213 to 244 (32 residues), 24.9 bits, see alignment (E = 8.2e-10) amino acids 249 to 282 (34 residues), 35.8 bits, see alignment (E = 3.2e-13) amino acids 338 to 374 (37 residues), 36.3 bits, see alignment (E = 2.2e-13) PF16168: AIDA" amino acids 323 to 385 (63 residues), 73.6 bits, see alignment 2.2e-24 amino acids 396 to 455 (60 residues), 47.3 bits, see alignment 3.5e-16 PF18883: AC_1" amino acids 496 to 599 (104 residues), 66.3 bits, see alignment E=4.8e-22 PF03797: Autotransporter" amino acids 676 to 924 (249 residues), 69.3 bits, see alignment E=9.5e-23

Best Hits

KEGG orthology group: K12687, antigen 43 (inferred from 97% identity to ecw:EcE24377A_3347)

Predicted SEED Role

"Antigen 43 precursor"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (948 amino acids)

>EFB2_00877 Antigen 43 (Escherichia fergusonii Becca)
MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAAVTSLPVLAADTVVQAG
ETVSGGALENHDNQIVFGTANGMTISTGLEYGPDNEANTGGQWIQNGGIANNTTVTGGGL
QRVNAGGSVSDTVIRAGGGQSLQGQAVNTTLNGGEQWVHEGGIATGTVINEKGWQAVKSG
AMATDTVVNTGAEGGPDAENGDTGQTVYGDAVRTTINKNGRQIVAAEGTANTTVVYAGGD
QTVHGHALDTTLNGGYQYVHNGGTASDTVVNSDGWQIIKEGGLADFTTVNQKGKLQVNAG
GTATNVTLKQGGALVTSTAATVTGSNRLGNFTVENGKADGVVLESGGRLDVLEGHSAQKT
RVDDGGTLAVSAGGKATGVTMTSGGALIADSGATVEGTNASGRFSIDGISGQASGLLLEN
GGSFTVNAGGQAGNTTVGHRGTLTLAAGGSLSGRTQLSKGASMVLNGDVVSTGDIVNAGE
IHFDTQMTQDAVLSRAVAKGASPVTFHKLTTSNLTGQGGTINMRVRLDGSNASDQLVING
GQATGKTWLAFTNVGNSNLGVATSGQGIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLN
RDSDEDWYLRSENAYRAEVPLYASMLTQAMDYDRILAGSRSHQTGVNGENNSIRLSIQGG
HLGHDNNGGIARGATPESSGSYGLVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDD
GSRAGTVRDDAGSLGGYLNLVHTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSL
ETGLPFSITDNLMLEPQLQYTWQGLSLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMN
FGKGTSSRDTLRDSAKHSVRELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSQN
GTSLDLQAGLEARVRENITLGVQAGYAHSVSGSSAEGYNGQATLNVTF