Protein Info for EFB2_00845 in Escherichia fergusonii Becca

Annotation: Secretin GspD 2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 686 signal peptide" amino acids 1 to 41 (41 residues), see Phobius details TIGR02517: type II secretion system protein D" amino acids 47 to 634 (588 residues), 551.3 bits, see alignment E=1.4e-169 PF21305: type_II_gspD_N0" amino acids 48 to 117 (70 residues), 82.4 bits, see alignment E=2.5e-27 PF03958: Secretin_N" amino acids 145 to 206 (62 residues), 51.2 bits, see alignment 1.8e-17 amino acids 211 to 277 (67 residues), 63.4 bits, see alignment E=2.9e-21 amino acids 284 to 362 (79 residues), 57.1 bits, see alignment E=2.6e-19 PF00263: Secretin" amino acids 465 to 626 (162 residues), 168.2 bits, see alignment E=1.9e-53

Best Hits

Swiss-Prot: 99% identical to GSPD2_ECOH1: Secretin GspD 2 (gspD2) from Escherichia coli O78:H11 (strain H10407 / ETEC)

KEGG orthology group: K02453, general secretion pathway protein D (inferred from 100% identity to ecv:APECO1_3458)

Predicted SEED Role

"General secretion pathway protein D"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (686 amino acids)

>EFB2_00845 Secretin GspD 2 (Escherichia fergusonii Becca)
MFWRDMTLSIWRKKTTGLKTKKRLLPLMLAAALCSSPVWAEEATFTANFKDTDLKSFIET
VGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEAQGYAVVPMENDVLKVVKS
SAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAPILRQMIDSAGSGNVVNYDPS
NVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNASASEIARVLESLTKNSGENQPA
TLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDSEMERSGNSQVFYLKYSKAEDLVDV
LKQVSGTLTAAKEEAEGTVGSGREVVSIAASKHSNALIVTAPQDIMQSLQSVIEQLDIRR
AQVHVEALIVEVAEGSNINFGVQWGSKDAGLMQFANGTQIPIGTLGAAISAAKPQKGSTV
ISENGATTINPDTNGDLSTLAQLLSGFSGTAVGVVKGDWMALVQAVKNDSSSNVLSTPSI
TTLDNQEAFFMVGQDVPVLTGSTVGSNNSNPFNTVERKKVGIMLKVTPQINEGNAVQMVI
EQEVSKVEGQTSLDVVFGERKLKTTVLANDGELIVLGGLMDDQAGESVAKVPLLGDIPLI
GNLFKSTADKKEKRNLMVFIRPTILRDGMAADGVSQRKYNYMRAEQIYRDEQGLSLMPHT
AQPILPAQNQALPPEVRAFLNAGRTR