Protein Info for EFB2_00735 in Escherichia fergusonii Becca

Annotation: Evolved beta-galactosidase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1030 PF02837: Glyco_hydro_2_N" amino acids 43 to 204 (162 residues), 161.3 bits, see alignment E=6.3e-51 PF22666: Glyco_hydro_2_N2" amino acids 112 to 179 (68 residues), 31.9 bits, see alignment 4.1e-11 PF00703: Glyco_hydro_2" amino acids 209 to 318 (110 residues), 72 bits, see alignment E=2e-23 PF02836: Glyco_hydro_2_C" amino acids 320 to 604 (285 residues), 415.3 bits, see alignment E=4e-128 PF16353: LacZ_4" amino acids 614 to 700 (87 residues), 68.1 bits, see alignment E=2e-22 PF02929: Bgal_small_N" amino acids 730 to 997 (268 residues), 227.2 bits, see alignment E=7.8e-71

Best Hits

Swiss-Prot: 98% identical to BGA2_ECOLI: Evolved beta-galactosidase subunit alpha (ebgA) from Escherichia coli (strain K12)

KEGG orthology group: K12111, evolved beta-galactosidase subunit alpha [EC: 3.2.1.23] (inferred from 100% identity to ecc:c3833)

Predicted SEED Role

"Evolved beta-D-galactosidase, alpha subunit" in subsystem Lactose utilization

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.23

Use Curated BLAST to search for 3.2.1.23

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1030 amino acids)

>EFB2_00735 Evolved beta-galactosidase subunit alpha (Escherichia fergusonii Becca)
MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLSLSGQWNFHFFDHPLQVP
EAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVPFVPSDNPTGAYQRIFTLS
DGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEFDISAMVKTGDNLLCVRVMQW
ADSTYVEDQDMWWSAGIFRDVYLIGKQLTHINDFTVRTDFDEAYCDATLSCEVVLENLAA
SPVVTTLEYTLFDGEHVVHSSTIDHLAIEKLTSASFAFTVEQPQQWSAESPYLYHLVMTL
KDADGNVLEVVPQRVGFRDIKVRDGLFWINNRYVMLHGVNRHDNDHRKGRAVGMDRVEKD
LQLMKQHNINSVRTAHYPNDPRFYELCDIYGLFVMAETDVESHGFANVGDISRITDDPQW
EKVYVERIVRHIHAQKNHPSIIIWSLGNESGYGCNIRAMYHAAKALDDTRLVHYEEDRDA
EVVDIISTMYTRVPLMNEFGEYPHPKPRIICEYAHAMGNGPGGLTEYQNVFYKHDCIQGH
YVWEWCDHGIQAQDDNGNVWYKFGGDYGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIA
PVKIHALDLTRGELKVENKLWFTTLDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQ
ITLPQLDAREAFLNITVTKDSRTRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLE
DDRLSCTVRGYNFAITFSKTSGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQ
PNHLQIMQEHLRDFVVEQSDDEVLLVSRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSG
ERYGDYPHIIPCIGFTMGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENY
PFPQNNGNRQHVRWTALTNRHGNGLLVVPQRPINFSAWRYTQENIHAAQHCNELQRSDDI
TLNLDHQLLGLGSNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFF
STNLHSENKQ