Protein Info for EFB2_00651 in Escherichia fergusonii Becca
Annotation: Argininosuccinate synthase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to ASSY_ECOLU: Argininosuccinate synthase (argG) from Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC)
KEGG orthology group: K01940, argininosuccinate synthase [EC: 6.3.4.5] (inferred from 100% identity to eco:b3172)Predicted SEED Role
"Argininosuccinate synthase (EC 6.3.4.5)" in subsystem Arginine Biosynthesis extended (EC 6.3.4.5)
MetaCyc Pathways
- superpathway of arginine and polyamine biosynthesis (16/17 steps found)
- L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
- L-arginine biosynthesis II (acetyl cycle) (9/10 steps found)
- L-arginine biosynthesis III (via N-acetyl-L-citrulline) (8/9 steps found)
- nitric oxide biosynthesis II (mammals) (2/3 steps found)
- urea cycle (3/5 steps found)
- canavanine biosynthesis (2/4 steps found)
- superpathway of L-citrulline metabolism (7/12 steps found)
- L-arginine biosynthesis IV (archaea) (4/9 steps found)
KEGG Metabolic Maps
- Alanine and aspartate metabolism
- Arginine and proline metabolism
- Urea cycle and metabolism of amino groups
Isozymes
No predicted isozymesUse Curated BLAST to search for 6.3.4.5
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (447 amino acids)
>EFB2_00651 Argininosuccinate synthase (Escherichia fergusonii Becca) MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTANLGQPDEEDYDAIPRRA MEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNTTPLGRAVTGTMLVAAMKE DGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDTDFIDELGGRHEMSEFMIACG FDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVNPIMGVKFWDESVKIPAEEVTVR FEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMSDQIENRIIEAKSRGIYEAPGMALL HIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQGRWFDSQALMLRDSLQRWVASQITGEV TLELRRGNDYSILNTVSENLTYKPERLTMEKGDSVFSPDDRIGQLTMRNLDITDTREKLF GYAKTGLLSSSAASGVPQVENLENKGQ