Protein Info for EFB2_00541 in Escherichia fergusonii Becca

Annotation: Threonylcarbamoyl-AMP synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 190 PF01300: Sua5_yciO_yrdC" amino acids 16 to 190 (175 residues), 159.1 bits, see alignment E=4.2e-51

Best Hits

Swiss-Prot: 100% identical to TSAC_ECOL6: Threonylcarbamoyl-AMP synthase (tsaC) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K07566, putative translation factor (inferred from 98% identity to ecd:ECDH10B_3457)

MetaCyc: 98% identical to L-threonylcarbamoyladenylate synthase (Escherichia coli K-12 substr. MG1655)
RXN-14569 [EC: 2.7.7.87]

Predicted SEED Role

"TsaC protein (YrdC domain) required for threonylcarbamoyladenosine t(6)A37 modification in tRNA"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.87

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (190 amino acids)

>EFB2_00541 Threonylcarbamoyl-AMP synthase (Escherichia fergusonii Becca)
MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVMRLLELKRRPVDKGLIL
IAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPATTPRWLTGRFDSLAVRVTD
HPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGAAFPVVSGETGGRLNPSEIRD
ALTGELFRQG