Protein Info for EFB2_00494 in Escherichia fergusonii Becca

Annotation: Putative type II secretion system protein H

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 169 transmembrane" amino acids 12 to 31 (20 residues), see Phobius details PF07963: N_methyl" amino acids 1 to 27 (27 residues), 32.5 bits, see alignment 4.5e-12 TIGR01708: type II secretion system protein H" amino acids 3 to 141 (139 residues), 64.9 bits, see alignment E=7.4e-22 TIGR02532: prepilin-type N-terminal cleavage/methylation domain" amino acids 4 to 27 (24 residues), 30.5 bits, see alignment 2.1e-11 PF12019: GspH" amino acids 44 to 146 (103 residues), 37.8 bits, see alignment E=2.3e-13

Best Hits

Swiss-Prot: 96% identical to GSPH_ECOLI: Putative type II secretion system protein H (gspH) from Escherichia coli (strain K12)

KEGG orthology group: K02457, general secretion pathway protein H (inferred from 100% identity to ecc:c4100)

MetaCyc: 96% identical to type II secretion system protein GspH (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"General secretion pathway protein H"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (169 amino acids)

>EFB2_00494 Putative type II secretion system protein H (Escherichia fergusonii Becca)
MNQQRGFTLLEMMLVLALVAITASVVLFTYGREDAANTRARETAARFTAALELAIDRATL
SGQPVGIHFSDSAWRIMVPGKTPSAWRWVPLQEDAADESKNDWGEELSIQLQPFKPDDSN
QPQVVILADGQITPFSLLMANTGTGEPLLTLVSSGSWPLDQTLARDTRP