Protein Info for ECD_04215 in Escherichia coli BL21

Annotation: DNA methyltransferase M

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 529 PF12161: HsdM_N" amino acids 6 to 108 (103 residues), 33.8 bits, see alignment E=5.1e-12 PF02384: N6_Mtase" amino acids 123 to 449 (327 residues), 317.1 bits, see alignment E=1.4e-98

Best Hits

Swiss-Prot: 97% identical to T1MK_ECOLI: Type I restriction enzyme EcoKI M protein (hsdM) from Escherichia coli (strain K12)

KEGG orthology group: K03427, type I restriction enzyme M protein [EC: 2.1.1.72] (inferred from 100% identity to ebr:ECB_04215)

MetaCyc: 97% identical to type I restriction enzyme EcoKI methylase subunit (Escherichia coli K-12 substr. MG1655)
Type I site-specific deoxyribonuclease. [EC: 3.1.21.3]

Predicted SEED Role

"Type I restriction-modification system, DNA-methyltransferase subunit M (EC 2.1.1.72)" in subsystem Restriction-Modification System (EC 2.1.1.72)

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.72, 3.1.21.3

Use Curated BLAST to search for 2.1.1.72 or 3.1.21.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (529 amino acids)

>ECD_04215 DNA methyltransferase M (Escherichia coli BL21)
MNNNDLVAKLWKLCDNLRDGGVSYQNYVNELASLLFLKMCKETGQEAEYLPEGYRWDDLK
SRIGQEQLQFYRNLLVHLGADNQKLVQAVFQNVNTTITQPKQLTELVSNMDSLDWYNGAH
GKSRDDFGDMYEGLLQKNANETKSGAGQYFTPRPLIKTIIHLLKPQPREVVQDPAAGTAG
FLIEADRYVKSQTNDLDDLDGDTQDFQIHRAFIGLELVPGTRRLALMNCLLHDIEGNLDH
GGAIRLGNTLGSDGENLPKAHIVATNPPFGSAAGTNITRTFVHPTSNKQLCFMQHIIETL
HPGGRAAVVVPDNVLFEGGKGTDIRRDLMDKCHLHTILRLPTGIFYAQGVKTNVLFFTKG
TVANPHQDKNCTDDVWVYDLRTNMPSFGKRTPFTDEHLQPFERVYGEDPHGLSPRSEGEW
SFNAEETEVADSEENKNTDQHLATSRWRKFTREWIRTTKSDSLDISWLKDKDSIDADNLP
EPDVLAAEAMGELVQALGELDALIRELGASDEADAQRQLLEEAFGGVKE