Protein Info for ECD_04033 in Escherichia coli BL21

Annotation: epoxyqueuosine reductase, cobalamine-stimulated; queosine biosynthesis

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 379 TIGR00276: epoxyqueuosine reductase" amino acids 14 to 348 (335 residues), 510.3 bits, see alignment E=1.1e-157 PF08331: QueG_DUF1730" amino acids 62 to 140 (79 residues), 84.7 bits, see alignment E=3.4e-28 PF13484: Fer4_16" amino acids 193 to 256 (64 residues), 80 bits, see alignment E=1.9e-26

Best Hits

Swiss-Prot: 100% identical to QUEG_ECOLI: Epoxyqueuosine reductase (queG) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to eco:b4166)

MetaCyc: 100% identical to epoxyqueuosine reductase (Escherichia coli K-12 substr. MG1655)
RXN-12104 [EC: 1.17.99.6]

Predicted SEED Role

No annotation

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.17.99.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (379 amino acids)

>ECD_04033 epoxyqueuosine reductase, cobalamine-stimulated; queosine biosynthesis (Escherichia coli BL21)
MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWLDKQYHGEMDWMARHGM
LRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVSRYALGRDYHKLLRNRLKK
LGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKHSLILNREAGSFFFLGELLVD
IPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARRCISYLTIELEGAIPEELRPLMG
NRIYGCDDCQLICPWNRYSQLTTEEDFSPRKPLHAPELIELFAWSEEKFLKVTEGSAIRR
IGHLRWLRNIAVALGNAPWDETILTALESRKGEHPLLDEHIAWAIAQQIERRNACIVEVQ
LPKKQRLVRVIEKGLPRDA