Protein Info for ECD_03916 in Escherichia coli BL21

Annotation: oxidative stress resistance protein; putative MATE family efflux pump; UV and mitomycin C inducible protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 459 transmembrane" amino acids 6 to 23 (18 residues), see Phobius details amino acids 29 to 49 (21 residues), see Phobius details amino acids 61 to 83 (23 residues), see Phobius details amino acids 103 to 124 (22 residues), see Phobius details amino acids 153 to 173 (21 residues), see Phobius details amino acids 180 to 199 (20 residues), see Phobius details amino acids 207 to 227 (21 residues), see Phobius details amino acids 259 to 278 (20 residues), see Phobius details amino acids 284 to 306 (23 residues), see Phobius details amino acids 338 to 359 (22 residues), see Phobius details amino acids 371 to 393 (23 residues), see Phobius details amino acids 404 to 422 (19 residues), see Phobius details amino acids 428 to 446 (19 residues), see Phobius details TIGR00797: MATE efflux family protein" amino acids 35 to 428 (394 residues), 347.2 bits, see alignment E=6e-108 PF01554: MatE" amino acids 35 to 196 (162 residues), 119.9 bits, see alignment E=8.7e-39 amino acids 259 to 412 (154 residues), 40.5 bits, see alignment E=2.3e-14 PF14667: Polysacc_synt_C" amino acids 156 to 245 (90 residues), 32.9 bits, see alignment E=6.7e-12

Best Hits

Swiss-Prot: 99% identical to DINF_ECOLI: DNA damage-inducible protein F (dinF) from Escherichia coli (strain K12)

KEGG orthology group: K03327, multidrug resistance protein, MATE family (inferred from 99% identity to eco:b4044)

Predicted SEED Role

"DNA-damage-inducible protein F"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (459 amino acids)

>ECD_03916 oxidative stress resistance protein; putative MATE family efflux pump; UV and mitomycin C inducible protein (Escherichia coli BL21)
MPPGVAVCFSSLFIRLVCMAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSP
VYLGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARAPV
ILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGISGEM
LKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTLLTFTAY
ALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGEHIIALLTS
LTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAAGFALTLLTLP
WLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT