Protein Info for ECD_03888 in Escherichia coli BL21

Annotation: isocitrate dehydrogenase kinase/phosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 578 PF20423: AceK_regulatory" amino acids 10 to 306 (297 residues), 368 bits, see alignment E=3.8e-114 PF06315: AceK_kinase" amino acids 310 to 564 (255 residues), 402.5 bits, see alignment E=8.1e-125

Best Hits

Swiss-Prot: 100% identical to ACEK_ECOLC: Isocitrate dehydrogenase kinase/phosphatase (aceK) from Escherichia coli (strain ATCC 8739 / DSM 1576 / Crooks)

KEGG orthology group: K00906, isocitrate dehydrogenase kinase/phosphatase [EC: 2.7.11.5 3.1.3.-] (inferred from 100% identity to eco:b4016)

MetaCyc: 100% identical to isocitrate dehydrogenase kinase/phosphatase (Escherichia coli K-12 substr. MG1655)
3.1.3.-; [Isocitrate dehydrogenase (NADP(+))] kinase. [EC: 2.7.11.5]

Predicted SEED Role

"Isocitrate dehydrogenase phosphatase (EC 2.7.11.5)/kinase (EC 3.1.3.-)" (EC 2.7.11.5, EC 3.1.3.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.-

Use Curated BLAST to search for 2.7.11.5 or 3.1.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (578 amino acids)

>ECD_03888 isocitrate dehydrogenase kinase/phosphatase (Escherichia coli BL21)
MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQQAMKNRIHLYDHHVGL
VVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESFFNSVYCRLFDHRSLTPER
LFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDLPLRLRWQNKSRDIHYIIRHL
TETLGTDNLAESHLQVANELFYRNKAAWLVGKLITSSGTLPFLLPIHQTDDGELFIDTCL
TTTAEASIVFGFARSYFMVYAPLPAALVEWLREILPGKTTAELYMAIGCQKHAKTESYRE
YLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRVFKVIKDKFAPQKEMSAAHVRACYQLV
KEHDRVGRMADTQEFENFVLEKRHISPALMELLLQEAAEKITDLGEQIVIRHLYIERRMV
PLNIWLEQVEGQQLRDAIEEYGNAIRQLAAANIFPGDMLFKNFGVTRHGRVVFYDYDEIC
YMTEVNFRDIPPPRYPEDELASEPWYSVSPGDVFPEEFRHWLCADPRIGPLFEEMHADLF
RADYWRALQNRIREGHVEDVYAYRRRQRFSVRYGEMLF