Protein Info for ECD_03708 in Escherichia coli BL21

Annotation: 5-methyltetrahydropteroyltriglutamate- homocysteine S-methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF08267: Meth_synt_1
  • amino acids 5 to 312 (308 residues), 417.4 bits, E=4.6e-129
• TIGR01371: 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase
  • amino acids 8 to 752 (745 residues), 1124.1 bits, E=0
• PF01717: Meth_synt_2
  • amino acids 153 to 221 (69 residues), 25.2 bits, E=1e-09
  • amino acids 426 to 749 (324 residues), 551.4 bits, E=7.2e-170

Best Hits

Swiss-Prot: 100% identical to METE_ECOL6: 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K00549, 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase [EC: 2.1.1.14] (inferred from 100% identity to ebr:ECB_03708)

MetaCyc: 100% identical to cobalamin-independent homocysteine transmethylase (Escherichia coli K-12 substr. MG1655)
5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase. [EC: 2.1.1.14]

Predicted SEED Role

"5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (EC 2.1.1.14)" in subsystem Methionine Biosynthesis (EC 2.1.1.14)

MetaCyc Pathways

• aspartate superpathway (24/25 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (18/18 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (9/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (9/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (8/8 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
• L-methionine biosynthesis I (5/5 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (10/12 steps found)
• S-adenosyl-L-methionine salvage I (4/4 steps found)
• L-methionine biosynthesis II (5/6 steps found)
• S-adenosyl-L-methionine salvage II (2/3 steps found)
• seleno-amino acid biosynthesis (plants) (3/5 steps found)
• L-methionine biosynthesis III (2/4 steps found)
• superpathway of seleno-compound metabolism (6/19 steps found)

KEGG Metabolic Maps

• Biosynthesis of plant hormones
• Methionine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.14

Protein Sequence (753 amino acids)

>ECD_03708 5-methyltetrahydropteroyltriglutamate- homocysteine S-methyltransferase (Escherichia coli BL21)
MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELRARHWDQQKQAGIDLLP
VGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRGRAPTGEPAAAAEMTKWFN
TNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHKVKPVLLGPVTWLWLGKVKGEQFDR
LSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELPQAWLDAYKPAYDALQGQVKLLL
TTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAELHKRLPSDWLLSVGLINGRNVWRAD
LTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSVETRLDAEVKSWFAFALQKCHELALLR
DALNSGDTAALAEWSAPIQARRHSTRVHNPAVEKRLAAITAQDSQRANVYEVRAEAQRAR
FKLPAWPTTTIGSFPQTTEIRTLRLDFKKGNLDANNYRTGIAEHIKQAIVEQERLGLDVL
VHGEAERNDMVEYFGEHLDGFVFTQNGWVQSYGSRCVKPPIVIGDVSRPAPITVEWAKYA
QSLTDKPVKGMLTGPVTILCWSFPREDVSRETIAKQIALALRDEVADLEAAGIGIIQIDE
PALREGLPLRRSDWDAYLQWGVEAFRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDAD
VITIETSRSDMELLESFEEFDYPNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERL
WVNPDCGLKTRGWPETRAALANMVQAAQNLRRG