Protein Info for ECD_03575 in Escherichia coli BL21

Annotation: D-galactonate dehydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 100% identical to DGOD2_ECOSM: D-galactonate dehydratase 2 (dgoD2) from Escherichia coli (strain SMS-3-5 / SECEC)

KEGG orthology group: K01684, galactonate dehydratase [EC: 4.2.1.6] (inferred from 100% identity to eco:b4478)

MetaCyc: 100% identical to D-galactonate dehydratase (Escherichia coli K-12 substr. MG1655)
Galactonate dehydratase. [EC: 4.2.1.140, 4.2.1.6]

Predicted SEED Role

"Galactonate dehydratase (EC 4.2.1.6)" in subsystem D-galactonate catabolism (EC 4.2.1.6)

MetaCyc Pathways

D-galactonate degradation (3/3 steps found)
Entner-Doudoroff pathway III (semi-phosphorylative) (6/9 steps found)
Entner-Doudoroff pathway II (non-phosphorylative) (5/9 steps found)

KEGG Metabolic Maps

Galactose metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.140 or 4.2.1.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (382 amino acids)

>ECD_03575 D-galactonate dehydratase (Escherichia coli BL21)
MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAAVHELGDYLIGQDPSRI
NDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPVWQLMGGLVRDKIKAYSWV
GGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAVDAAVNTVAQIREAFGNQIEF
GLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAEYYPKLAAQTHIPLAAGERMFSR
FDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAEAYDVTLAPHCPLGPIALAACLHID
FVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFSMVGGFFKPLTKPGLGVEIDEAKVIEF
SKNAPDWRNPLWRHEDNSVAEW