Protein Info for ECD_03269 in Escherichia coli BL21

Annotation: maltodextrin phosphorylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 797 TIGR02093: glycogen/starch/alpha-glucan phosphorylases" amino acids 9 to 794 (786 residues), 1093.6 bits, see alignment E=0 PF00343: Phosphorylase" amino acids 93 to 794 (702 residues), 1034.9 bits, see alignment E=0

Best Hits

Swiss-Prot: 100% identical to PHSM_ECOLI: Maltodextrin phosphorylase (malP) from Escherichia coli (strain K12)

KEGG orthology group: K00688, starch phosphorylase [EC: 2.4.1.1] (inferred from 100% identity to eoi:ECO111_4226)

MetaCyc: 100% identical to maltodextrin phosphorylase (Escherichia coli K-12 substr. MG1655)
Phosphorylase. [EC: 2.4.1.1]; 2.4.1.1 [EC: 2.4.1.1]; 2.4.1.1 [EC: 2.4.1.1]; 2.4.1.1 [EC: 2.4.1.1]; 2.4.1.1 [EC: 2.4.1.1]

Predicted SEED Role

"Maltodextrin phosphorylase (EC 2.4.1.1)" in subsystem Glycogen metabolism or Maltose and Maltodextrin Utilization (EC 2.4.1.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.1

Use Curated BLAST to search for 2.4.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (797 amino acids)

>ECD_03269 maltodextrin phosphorylase (Escherichia coli BL21)
MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALAEMLRAQPFAKPVANQR
HVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDLLEEEIDPALGNGGLGRLA
ACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPDDWHRSNYPWFRHNEALDVQV
GIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPLRLWQATHAHPFDLTKFNDGDFL
RAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQCACSVADILRRHHLAGRKLHELAD
YEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAWAITSKTFAYTNHTLMPEALERWDVKL
VKGLLPRHMQIINEINTRFKTLVEKTWPGDEKVWAKLAVVHDKQVHMANLCVVGGFAVNG
VAALHSDLVVKDLFPEYHQLWPNKFHNVTNGITPRRWIKQCNPALAALLDKSLKKEWAND
LDQLINLEKFADDAKFRQQYREIKQANKVRLAEFVKVRTGIEINPQAIFDIQIKRLHEYK
RQHLNLLHILALYKEIRENPQADRVPRVFLFGAKAAPGYYLAKNIIFAINKVADVINNDP
LVGDKLKVVFLPDYCVSAAEKLIPAADISEQISTAGKEASGTGNMKLALNGALTVGTLDG
ANVEIAEKVGEENIFIFGHTVEQVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGD
KHAFDQMLHSIGKQGGDPYLVMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFS
SDRSIRDYQARIWQAKR