Protein Info for ECD_03233 in Escherichia coli BL21

Annotation: putative pyridoxal 5'-phosphate binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 387 PF01168: Ala_racemase_N" amino acids 33 to 264 (232 residues), 76.5 bits, see alignment E=2.7e-25 PF21279: YhfX-like_C" amino acids 278 to 374 (97 residues), 129.7 bits, see alignment E=5e-42

Best Hits

Swiss-Prot: 97% identical to YHFX_ECOLI: Uncharacterized protein YhfX (yhfX) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to ecl:EcolC_0332)

Predicted SEED Role

"Protein containing an Alanine Racemase Domain"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (387 amino acids)

>ECD_03233 putative pyridoxal 5'-phosphate binding protein (Escherichia coli BL21)
MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKRLIETARLYGIELYLMT
KQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQGHLVQIPCHQVADAVEQG
TDVITVFTLDKAREVSAAAVKAGRVQFVLLKVYSDDDFLYPGQESGFVQHSLHEVVAEIK
KLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLIQARDQLAKSGIALEQLNAPSATSCT
SLPLLAQYGVTHAEPGHALTGTIPANQQGDQPERIAMLWLSEISHHFRGDSYCYGGGYYR
RGHAQHALVFTPENQKITETNLKTVDDSSIDYTLPLAGEFPVSSAVVLCFRTQIFVTRSD
VVLVSGIHRGEPEIVGRYDSLGNSLGA