Protein Info for ECD_02993 in Escherichia coli BL21

Annotation: D-galactarate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 523 TIGR03248: galactarate dehydratase" amino acids 15 to 523 (509 residues), 940.4 bits, see alignment E=1.1e-287 PF08666: SAF" amino acids 22 to 92 (71 residues), 39.8 bits, see alignment E=8e-14 PF04295: GD_AH_second" amino acids 122 to 270 (149 residues), 121.6 bits, see alignment E=4e-39 PF20629: GD_AH_C" amino acids 279 to 520 (242 residues), 315.3 bits, see alignment E=3.7e-98

Best Hits

Swiss-Prot: 100% identical to GARD_ECOLI: Galactarate dehydratase (L-threo-forming) (garD) from Escherichia coli (strain K12)

KEGG orthology group: K01708, galactarate dehydratase [EC: 4.2.1.42] (inferred from 100% identity to eco:b3128)

MetaCyc: 100% identical to GarD (Escherichia coli K-12 substr. MG1655)
Galactarate dehydratase. [EC: 4.2.1.42]

Predicted SEED Role

"D-galactarate dehydratase (EC 4.2.1.42)" in subsystem D-Galacturonate and D-Glucuronate Utilization or D-galactarate, D-glucarate and D-glycerate catabolism (EC 4.2.1.42)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.42

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (523 amino acids)

>ECD_02993 D-galactarate dehydrogenase (Escherichia coli BL21)
MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLELIEHIPQGHKVALLDI
PANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTLPLATKVPEPLPPLEGYTF
EGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDLLPKYPNVDGVVGLNHLYGCG
VAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKLQPERLLTGTDDVQAIPVESASI
VSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRETCPASELVVGMQCGGSDAFSGVTAN
PAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTPRAVNEEVGKRLLEEMEWYDNYLNMGK
TDRSANPSPGNKKGGLANVVEKALGSIAKSGKSAIVEVLSPGQRPTKRGLIYAATPASDF
VCGTQQVASGITVQVFTTGRGTPYGLMAVPVIKMATRTELANRWFDLMDINAGTIATGEE
TIEEVGWKLFHFILDVASGKKKTFSDQWGLHNQLAVFNPAPVT