Protein Info for ECD_02962 in Escherichia coli BL21

Annotation: hexuronate transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 432 signal peptide" amino acids 1 to 32 (32 residues), see Phobius details transmembrane" amino acids 47 to 69 (23 residues), see Phobius details amino acids 76 to 96 (21 residues), see Phobius details amino acids 102 to 122 (21 residues), see Phobius details amino acids 135 to 159 (25 residues), see Phobius details amino acids 165 to 184 (20 residues), see Phobius details amino acids 237 to 255 (19 residues), see Phobius details amino acids 266 to 286 (21 residues), see Phobius details amino acids 300 to 322 (23 residues), see Phobius details amino acids 327 to 349 (23 residues), see Phobius details amino acids 361 to 384 (24 residues), see Phobius details amino acids 390 to 412 (23 residues), see Phobius details PF07690: MFS_1" amino acids 16 to 293 (278 residues), 163.8 bits, see alignment E=2.8e-52 amino acids 301 to 417 (117 residues), 36.6 bits, see alignment E=1.3e-13 TIGR00893: D-galactonate transporter" amino acids 17 to 411 (395 residues), 426.8 bits, see alignment E=4.2e-132

Best Hits

Swiss-Prot: 100% identical to EXUT_ECOLI: Hexuronate transporter (exuT) from Escherichia coli (strain K12)

KEGG orthology group: K08191, MFS transporter, ACS family, hexuronate transporter (inferred from 100% identity to eco:b3093)

MetaCyc: 100% identical to hexuronate transporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-123; TRANS-RXN-35

Predicted SEED Role

"Hexuronate transporter" in subsystem Alginate metabolism or D-Galacturonate and D-Glucuronate Utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (432 amino acids)

>ECD_02962 hexuronate transporter (Escherichia coli BL21)
MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNISTQQYSYIIAAYSAAYTV
MQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLAIARGAVGAAEAAMIPAGL
KASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVMHSWQMAFIISGALSFIWAMA
WLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKMSVGQILRNRQFWGIALPRFLAE
PAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFADLGCILGGYLPPLFQRWFGVNLIV
SRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCIGGFAHQALSGALITLSSDVFGRNEVA
TANGLTGMSAWLASTLFALVVGALADTIGFSPLFAVLAVFDLLGALVIWTVLQNKPAIEV
AQETHNDPAPQH