Protein Info for ECD_02881 in Escherichia coli BL21
Annotation: cystathionine beta-lyase, PLP-dependent
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 99% identical to METC_ECOLI: Cystathionine beta-lyase MetC (metC) from Escherichia coli (strain K12)
KEGG orthology group: K01760, cystathionine beta-lyase [EC: 4.4.1.8] (inferred from 99% identity to eco:b3008)MetaCyc: 99% identical to cystathionine beta-lyase / L-cysteine desulfhydrase / alanine racemase (Escherichia coli K-12 substr. MG1655)
Alanine racemase. [EC: 5.1.1.1, 5.1.1.10]; Cysteine-S-conjugate beta-lyase. [EC: 5.1.1.1, 5.1.1.10, 4.4.1.13]; Cystathionine gamma-lyase. [EC: 5.1.1.1, 5.1.1.10, 4.4.1.13, 4.4.1.1, 4.4.1.28]
Predicted SEED Role
"Cystathionine beta-lyase (EC 4.4.1.8)" in subsystem Methionine Biosynthesis (EC 4.4.1.8)
MetaCyc Pathways
- aspartate superpathway (24/25 steps found)
- superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (18/18 steps found)
- superpathway of branched chain amino acid biosynthesis (17/17 steps found)
- superpathway of L-isoleucine biosynthesis I (13/13 steps found)
- superpathway of L-threonine metabolism (16/18 steps found)
- superpathway of S-adenosyl-L-methionine biosynthesis (9/9 steps found)
- superpathway of L-methionine biosynthesis (transsulfuration) (9/9 steps found)
- superpathway of L-homoserine and L-methionine biosynthesis (8/8 steps found)
- superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
- L-isoleucine biosynthesis I (from threonine) (7/7 steps found)
- L-threonine degradation I (6/6 steps found)
- L-methionine biosynthesis I (5/5 steps found)
- D-serine degradation (3/3 steps found)
- L-serine degradation (3/3 steps found)
- L-tryptophan degradation II (via pyruvate) (3/3 steps found)
- L-methionine biosynthesis II (5/6 steps found)
- L-alanine degradation I (2/2 steps found)
- alanine racemization (1/1 steps found)
- anaerobic energy metabolism (invertebrates, cytosol) (5/7 steps found)
- L-cysteine degradation II (2/3 steps found)
- L-methionine degradation II (2/3 steps found)
- superpathway of anaerobic energy metabolism (invertebrates) (12/17 steps found)
- purine nucleobases degradation II (anaerobic) (17/24 steps found)
- seleno-amino acid biosynthesis (plants) (3/5 steps found)
- homocysteine and cysteine interconversion (2/4 steps found)
- L-cysteine biosynthesis VI (reverse transsulfuration) (4/7 steps found)
- glycine betaine degradation III (4/7 steps found)
- vancomycin resistance II (1/3 steps found)
- superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (6/10 steps found)
- felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis (2/5 steps found)
- L-mimosine degradation (4/8 steps found)
- glycine betaine degradation I (4/8 steps found)
- superpathway of L-cysteine biosynthesis (fungi) (2/6 steps found)
- superpathway of L-cysteine biosynthesis (mammalian) (1/5 steps found)
- glutathione-mediated detoxification I (3/8 steps found)
- L-arginine degradation XIV (oxidative Stickland reaction) (1/6 steps found)
- hydrogen sulfide biosynthesis II (mammalian) (1/6 steps found)
- L-lysine degradation V (2/9 steps found)
- ansatrienin biosynthesis (1/9 steps found)
- glutathione-mediated detoxification II (1/9 steps found)
- hypoglycin biosynthesis (4/14 steps found)
- superpathway of L-methionine salvage and degradation (5/16 steps found)
- superpathway of seleno-compound metabolism (6/19 steps found)
- cyclosporin A biosynthesis (2/15 steps found)
- superpathway of L-lysine degradation (15/43 steps found)
KEGG Metabolic Maps
- Alanine and aspartate metabolism
- Biosynthesis of plant hormones
- Cysteine metabolism
- D-Alanine metabolism
- D-Arginine and D-ornithine metabolism
- D-Glutamine and D-glutamate metabolism
- Glycine, serine and threonine metabolism
- Methionine metabolism
- Nitrogen metabolism
- Selenoamino acid metabolism
- Sulfur metabolism
Isozymes
Compare fitness of predicted isozymes for: 4.4.1.8, 5.1.1.1
Use Curated BLAST to search for 4.4.1.1 or 4.4.1.13 or 4.4.1.28 or 4.4.1.8 or 5.1.1.1 or 5.1.1.10
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (395 amino acids)
>ECD_02881 cystathionine beta-lyase, PLP-dependent (Escherichia coli BL21) MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKHATRNRANGELFYGRRG TLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGDHVLMTNTAYEPSQDFCSK ILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSITMEVHDVPAIVAAVRSVVPDA IIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDAMIGTAVCNARCWEQLRENAYLM GQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEWLAEHPQVARVNHPALPGSKGHEFW KRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLFSMAYSWGGYESLILANQPEHIAAIRP QGEIDFSGTLIRLHIGLEDVDDLIADLDAGFARIV