Protein Info for ECD_02831 in Escherichia coli BL21

Annotation: putative type II secretion protein GspK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 311 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details PF21687: T2SSK_1st" amino acids 89 to 195 (107 residues), 117.5 bits, see alignment E=4.6e-38 PF03934: T2SSK" amino acids 201 to 269 (69 residues), 66.9 bits, see alignment E=1.6e-22

Best Hits

KEGG orthology group: K02460, general secretion pathway protein K (inferred from 100% identity to ebr:ECB_02831)

Predicted SEED Role

"General secretion pathway protein K"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (311 amino acids)

>ECD_02831 putative type II secretion protein GspK (Escherichia coli BL21)
MLVLLAVMMLVTITLSGRMQQQLGRTRSQQEYQQALWYSASAESLALSALSLSLKNEKRV
HLAQPWASGPRFFPLPQGQIAVTLRDAQACFNLNALAQPTTASRPIAVQQLIALITRLDV
PAYRAELIAESLWEFIDEDRSVQTRLGREDSEYLARSVPFYAANQPLADISEMRVVQGMD
AGLYQKLKPLVCALPMTRQQININTLDVTQSVILEALFDPWLSPVQARVLLQQRPAKGWE
DVDQFLAQPLLADVDERTKKQLKTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHF
SVLWHQTGESE