Protein Info for ECD_02298 in Escherichia coli BL21
Annotation: glucokinase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to GLK_ECOLU: Glucokinase (glk) from Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC)
KEGG orthology group: K00845, glucokinase [EC: 2.7.1.2] (inferred from 100% identity to eco:b2388)MetaCyc: 100% identical to glucokinase (Escherichia coli K-12 substr. MG1655)
Glucosamine kinase. [EC: 2.7.1.8]; Hexokinase. [EC: 2.7.1.8, 2.7.1.1, 2.7.1.2]
Predicted SEED Role
"Glucokinase (EC 2.7.1.2)" in subsystem Entner-Doudoroff Pathway or Glycolysis and Gluconeogenesis (EC 2.7.1.2)
MetaCyc Pathways
- homolactic fermentation (12/12 steps found)
- glycolysis III (from glucose) (11/11 steps found)
- glycogen degradation I (8/8 steps found)
- Bifidobacterium shunt (13/15 steps found)
- heterolactic fermentation (15/18 steps found)
- glucose and glucose-1-phosphate degradation (5/5 steps found)
- trehalose degradation IV (3/3 steps found)
- trehalose degradation I (low osmolarity) (2/2 steps found)
- trehalose degradation II (cytosolic) (2/2 steps found)
- 1,3-propanediol biosynthesis (engineered) (7/9 steps found)
- sucrose biosynthesis II (6/8 steps found)
- sucrose degradation III (sucrose invertase) (3/4 steps found)
- GDP-α-D-glucose biosynthesis (2/3 steps found)
- trehalose degradation V (2/3 steps found)
- UDP-N-acetyl-D-glucosamine biosynthesis II (4/6 steps found)
- glycogen degradation II (4/6 steps found)
- chitin biosynthesis (6/9 steps found)
- UDP-N-acetyl-D-galactosamine biosynthesis II (4/7 steps found)
- chitin derivatives degradation (3/8 steps found)
KEGG Metabolic Maps
- Aminosugars metabolism
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Fructose and mannose metabolism
- Galactose metabolism
- Glycolysis / Gluconeogenesis
- Nucleotide sugars metabolism
- Starch and sucrose metabolism
- Streptomycin biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 2.7.1.2
Use Curated BLAST to search for 2.7.1.1 or 2.7.1.2 or 2.7.1.8
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (321 amino acids)
>ECD_02298 glucokinase (Escherichia coli BL21) MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAVIRVYLEEHKVEVKDGC IAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFTAVSMAIPMLKKEHLIQFG GAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGHVDFAPNSEEEAIILEILRAE IGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDITERALADSCTDCRRALSLFCVI MGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASGFRAAFEDKGRFKEYVHDIPVYLIV HDNPGLLGSGAHLRQTLGHIL