Protein Info for ECD_02249 in Escherichia coli BL21

Annotation: fused 5-methylaminomethyl-2-thiouridine-forming enzyme methyltransferase and FAD-dependent demodification enzyme

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 668 transmembrane" amino acids 267 to 284 (18 residues), see Phobius details PF05430: Methyltransf_30" amino acids 118 to 244 (127 residues), 170 bits, see alignment E=2e-54 TIGR03197: tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC, C-terminal domain" amino acids 267 to 662 (396 residues), 518.4 bits, see alignment E=7.1e-160 PF01266: DAO" amino acids 267 to 632 (366 residues), 177.2 bits, see alignment E=7e-56

Best Hits

Swiss-Prot: 100% identical to MNMC_ECOLI: tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC (mnmC) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to eco:b2324)

MetaCyc: 100% identical to fused tRNA 5-aminomethyl-2-thiouridylate methyltransferase and tRNA 5-aminomethyl-2-thiouridylate synthase (Escherichia coli K-12 substr. MG1655)
tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase. [EC: 2.1.1.61]; RXN0-7081 [EC: 2.1.1.61]

Predicted SEED Role

"5-methylaminomethyl-2-thiouridine-forming enzyme mnmC"

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.61

Use Curated BLAST to search for 2.1.1.61

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (668 amino acids)

>ECD_02249 fused 5-methylaminomethyl-2-thiouridine-forming enzyme methyltransferase and FAD-dependent demodification enzyme (Escherichia coli BL21)
MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLGGNQLEVRFPEHPHPLF
VVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEKFPLTRADLALAHQHWPEL
APWAEQLQAQWPMPLPGCHRLLLDAGRVTLDLWFGDINELTSQLDDSLNQKVDAWFLDGF
APAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRRGLQDAGFTMQKRKGFGRKREML
CGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIASALLSLALLRRGWQVTLYCADEAPA
LGASGNRQGALYPLLSKHDEALNRFFSNAFTFARRFYDQLPVKFDHDWCGVTQLGWDEKS
QHKIAQMLSMDLPAELAVAVEANAVEQITGVATNCSGITYPQGGWLCPAELTRNVLELAQ
QQGLQIYYQYQLQNFSRKDDCWLLNFAGDQQATHSVVVLANGHQISRFSQTSTLPVYSVA
GQVSHIPTTPELAELKQVLCYDGYLTPQNPANQHHCIGASYHRGSEDTAYSEDDQQQNRQ
RLIDCFPQAQWAKEVDVSDKEARCGVRCATRDHLPMVGNVPDYEATLVEYASLAEQKDEA
VSAPVFDDLFMFAALGSRGLCSAPLCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLL
KGKAVKAG