Protein Info for ECD_02208 in Escherichia coli BL21

Annotation: NADH:ubiquinone oxidoreductase, chain G

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 908 PF13510: Fer2_4" amino acids 3 to 77 (75 residues), 44.1 bits, see alignment E=6e-15 TIGR01973: NADH dehydrogenase (quinone), G subunit" amino acids 4 to 655 (652 residues), 683.5 bits, see alignment E=1.7e-209 PF00111: Fer2" amino acids 4 to 71 (68 residues), 31 bits, see alignment 6.7e-11 PF10588: NADH-G_4Fe-4S_3" amino acids 88 to 124 (37 residues), 56.9 bits, see alignment (E = 4.2e-19) PF22117: Fer4_Nqo3" amino acids 141 to 207 (67 residues), 93.8 bits, see alignment 2.1e-30 PF04879: Molybdop_Fe4S4" amino acids 221 to 274 (54 residues), 49.2 bits, see alignment 1.5e-16 PF00384: Molybdopterin" amino acids 485 to 637 (153 residues), 44.7 bits, see alignment E=3.4e-15 PF01568: Molydop_binding" amino acids 827 to 889 (63 residues), 29.5 bits, see alignment 2.3e-10

Best Hits

Swiss-Prot: 100% identical to NUOG_ECO57: NADH-quinone oxidoreductase subunit G (nuoG) from Escherichia coli O157:H7

KEGG orthology group: K00336, NADH dehydrogenase I subunit G [EC: 1.6.5.3] (inferred from 100% identity to ebr:ECB_02208)

MetaCyc: 66% identical to NADH-quinone oxidoreductase subunit G (Pseudomonas putida KT2440)
NADH-DEHYDROG-A-RXN [EC: 7.1.1.2]

Predicted SEED Role

"NADH-ubiquinone oxidoreductase chain G (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3

Use Curated BLAST to search for 1.6.5.3 or 7.1.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (908 amino acids)

>ECD_02208 NADH:ubiquinone oxidoreductase, chain G (Escherichia coli BL21)
MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGACRQCAVKQYQNAEDTR
GRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHDCPVCEEGGNCHLQDMTVM
TGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRYYKDYAGGTDLGVYGAHDNVY
FGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRKWDMQFAPSICQQCSIGCNISPG
ERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLKDRPRQPVQRRGDDFITLNAEQAMQ
GAADILRQSKKVIGIGSPRASVESNFALRELVGEENFYTGIAHGEQERLQLALKVLREGG
IYTPALREIESYDAVLVLGEDVTQTGARVALAVRQAVKGKAREMAAAQKVADWQIAAILN
IGQRAKHPLFVTNVDDTRLDDIAAWTYRAPVEDQARLGFAIAHALDNSAPAVDGIEPELQ
SKIDVIVQALAGAKKPLIISGTNAGSLEVIQAAANVAKALKGRGADVGITMIARSVNSMG
LGIMGGGSLEEALTELETGRADAVVVLENDLHRHASATRVNAALAKAPLVMVVDHQRTAI
MENAHLVLSAASFAESDGTVINNEGRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLL
SREVDWTQLDHVIDAVVAKIPELAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANIS
VHEPRQPQDIDTMFTFSMEGNNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGD
PGVRLFETSENGLDYFTSVPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYI
KLNPADAAKLGVNAGTRVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHL
EDLKEAQQ