Protein Info for ECD_02145 in Escherichia coli BL21

Annotation: hybrid sensory kinase in two-component regulatory system with RcsB and YojN

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 949 signal peptide" amino acids 1 to 41 (41 residues), see Phobius details transmembrane" amino acids 314 to 334 (21 residues), see Phobius details PF00512: HisKA" amino acids 470 to 534 (65 residues), 64.2 bits, see alignment 1.9e-21 PF02518: HATPase_c" amino acids 581 to 690 (110 residues), 109.8 bits, see alignment E=2.1e-35 PF09456: RcsC" amino acids 709 to 799 (91 residues), 129.3 bits, see alignment E=8e-42 PF00072: Response_reg" amino acids 827 to 936 (110 residues), 102.3 bits, see alignment E=3.5e-33

Best Hits

Swiss-Prot: 100% identical to RCSC_ECOLI: Sensor histidine kinase RcsC (rcsC) from Escherichia coli (strain K12)

KEGG orthology group: K07677, two-component system, NarL family, capsular synthesis sensor histidine kinase RcsC [EC: 2.7.13.3] (inferred from 100% identity to ecx:EcHS_A2359)

MetaCyc: 100% identical to histidine kinase RcsC (Escherichia coli K-12 substr. MG1655)
Histidine kinase. [EC: 2.7.13.3]

Predicted SEED Role

"Two-component sensor protein RcsC (EC 2.7.3.-)" (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (949 amino acids)

>ECD_02145 hybrid sensory kinase in two-component regulatory system with RcsB and YojN (Escherichia coli BL21)
MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQRESEIRQEFNLSSDQAQ
RFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAFEPLFADSDCSAMSNTWRG
SLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGLRDMPVERDTALKALHERINK
YRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANRLQALLGVEQTIRMENFFLPGTL
PMGVTILDENGHTLISLTGPESKIKGDPRWMQERSWFGYTEGFRELVLKKNLPPSSLSIV
YSVPVDKVLERIRMLILNAILLNVLAGAALFTLARMYERRIFIPAESDALRLEEHEQFNR
KIVASAPVGICILRTADGVNILSNELAHTYLNMLTHEDRQRLTQIICGQQVNFVDVLTSN
NTNLQISFVHSRYRNENVAICVLVDVSSRVKMEESLQEMAQAAEQASQSKSMFLATVSHE
LRTPLYGIIGNLDLLQTKELPKGVDRLVTAMNNSSSLLLKIISDILDFSKIESEQLKIEP
REFSPREVMNHITANYLPLVVRKQLGLYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKF
TDTGCIVLHVRADGDYLSIRVRDTGVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAI
CEKLISMMDGDISVDSEPGMGSQFTVRIPLYGAQYPQKKGVEGLSGKRCWLSVRNASLCQ
FLETSLQRSGIVVTTYEGQEPTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGE
WVHSVAAPHELPALLARIYLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLL
ADQLGSLGYQCKTANDGVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVI
GVTANALAEEKQRCLESGMDSCLSKPVTLDVIKQTLTLYAERVRKSRDS