Protein Info for ECD_01827 in Escherichia coli BL21

Annotation: murein DD-endopeptidase, space-maker hydrolase, septation protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 440 transmembrane" amino acids 21 to 40 (20 residues), see Phobius details PF08525: OapA_N" amino acids 12 to 39 (28 residues), 36.1 bits, see alignment (E = 1.1e-12) PF04225: OapA" amino acids 96 to 172 (77 residues), 29.8 bits, see alignment E=1.3e-10 PF01476: LysM" amino acids 98 to 141 (44 residues), 31.4 bits, see alignment 3.7e-11 PF19425: Csd3_N2" amino acids 180 to 301 (122 residues), 220.3 bits, see alignment E=1.3e-69 PF01551: Peptidase_M23" amino acids 313 to 407 (95 residues), 121.4 bits, see alignment E=3.8e-39

Best Hits

Swiss-Prot: 100% identical to MEPM_SHIFL: Murein DD-endopeptidase MepM (mepM) from Shigella flexneri

KEGG orthology group: K01417, [EC: 3.4.24.-] (inferred from 100% identity to eco:b1856)

MetaCyc: 100% identical to peptidoglycan endopeptidase MepM (Escherichia coli K-12 substr. MG1655)
3.4.-.-; 3.4.-.-

Predicted SEED Role

"Cell wall endopeptidase, family M23/M37"

Isozymes

Compare fitness of predicted isozymes for: 3.4.24.-

Use Curated BLAST to search for 3.4.24.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (440 amino acids)

>ECD_01827 murein DD-endopeptidase, space-maker hydrolase, septation protein (Escherichia coli BL21)
MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRDATPIVKTIELEQNEIR
SLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVSTGDTLSSILNQYGIDMGDI
TQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRETRTYDRTAANGFKMTSEMQQ
GEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQWQMDFRKLKKGDEFAVLMSREM
LDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRNGTGLAKGFLRFPTAKQFRISSNFN
PRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGEVVVAKRSGAAGYYVAIRHGRSYTTRY
MHLRKILVKPGQKVKRGDRIALSGNTGRSTGPHLHYEVWINQQAVNPLTAKLPRTEGLTG
SDRREFLAQAKEIVPQLRFD