Protein Info for ECD_01752 in Escherichia coli BL21

Annotation: protein kinase, endogenous substrate unidentified; autokinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 602 PF08298: AAA_PrkA" amino acids 2 to 338 (337 residues), 587.8 bits, see alignment E=7.6e-181 PF06798: PrkA" amino acids 340 to 594 (255 residues), 248.6 bits, see alignment E=8.4e-78

Best Hits

Swiss-Prot: 100% identical to YEAG_ECO57: Uncharacterized protein YeaG (yeaG) from Escherichia coli O157:H7

KEGG orthology group: K07180, serine protein kinase (inferred from 100% identity to ecg:E2348C_1910)

MetaCyc: 100% identical to protein kinase YeaG (Escherichia coli K-12 substr. MG1655)
2.7.11.d,2.7.11.11,2.7.12.1,2.7.11.12,2.7.11.17,2.7.11.1,2.7.11.13,2.7.11.22,2.7.11.21,2.7.11.34,2.7.11.31 [EC: 2.7.11.1, 2.7.11.11, 2.7.11.12, 2.7.11.13, 2.7.11.17, 2.7.11.21, 2.7.11.22, 2.7.11.31, 2.7.11.34, 2.7.11.d, 2.7.12.1]

Predicted SEED Role

No annotation

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.11.1 or 2.7.11.11 or 2.7.11.12 or 2.7.11.13 or 2.7.11.17 or 2.7.11.21 or 2.7.11.22 or 2.7.11.31 or 2.7.11.34 or 2.7.11.d or 2.7.12.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (602 amino acids)

>ECD_01752 protein kinase, endogenous substrate unidentified; autokinase (Escherichia coli BL21)
MMAIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQILE
KEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDENNQDI
SALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLLTATQEG
NYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLRISEEIKIY
EKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGESLKDTDPKAKS
YQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVLEQQIEREQFPQE
QAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVTYADFWIQDQEYRDP
DTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARANNSGRNPNWTSYEKLRT
VIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKGYTRKQVRLLCEWYLRVRK
SS