Protein Info for ECD_01293 in Escherichia coli BL21

Annotation: putative family 65 glycosyl hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 755 PF03636: Glyco_hydro_65N" amino acids 9 to 237 (229 residues), 170.6 bits, see alignment E=7.2e-54 PF03632: Glyco_hydro_65m" amino acids 298 to 660 (363 residues), 494.9 bits, see alignment E=2.3e-152 PF03633: Glyco_hydro_65C" amino acids 672 to 729 (58 residues), 52.9 bits, see alignment 3.6e-18

Best Hits

Swiss-Prot: 99% identical to KOJP_ECOLI: Kojibiose phosphorylase (ycjT) from Escherichia coli (strain K12)

KEGG orthology group: K04844, hypothetical glycosyl hydrolase [EC: 3.2.1.-] (inferred from 99% identity to eco:b1316)

MetaCyc: 99% identical to kojibiose phosphorylase (Escherichia coli K-12 substr. MG1655)
Kojibiose phosphorylase. [EC: 2.4.1.230]

Predicted SEED Role

"Maltose phosphorylase (EC 2.4.1.8) / Trehalose phosphorylase (EC 2.4.1.64)" in subsystem Maltose and Maltodextrin Utilization or Trehalose Biosynthesis or Trehalose Uptake and Utilization (EC 2.4.1.64, EC 2.4.1.8)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.-

Use Curated BLAST to search for 2.4.1.230 or 2.4.1.64 or 2.4.1.8 or 3.2.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (755 amino acids)

>ECD_01293 putative family 65 glycosyl hydrolase (Escherichia coli BL21)
MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQTRGMYLAGLYHRAGKG
EINELVNLPDVVGMEIAINGEVFSLSHEAWQRELDFASGELRRNVVWRTSNGAGYTIASR
RFVSADQLPLIALEITITPLDADTSVLISTGIDATQTNHGRQHLDETQVRVFGQHLMQGI
YTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTSAQLHAGETVTLQKLVWIDWRDD
RQAALDEWGSASLRQLEMCAQQSYDQLLAASTENWRQWWQKRRITVNGGDAHDQQALDYA
LYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWDTEVFLLPFHLFSDPTVARSLLRYRWH
NLPGAQEKARRNGWQGALFPWESARSGEEETPEFAAINIRTGLRQKVASAQAEHHLVADI
AWAVIQYWQTTGDESFIAHEGMALLLETAKFWISRAVRVNDRLEIHDVIGPDEYTEHVNN
NAYTSYMARYNVQQALNIARQFGCSDDAFIHRAEMFLKELWMPEIQPDGVLPQDDSFMAK
PAINLAKYKAAAGKQTILLDYSRAEVNEMQILKQADVVMLNYMLPEQFSAASCLANLQFY
EPRTIHDSSLSKAIHGIVAARCGLLTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIW
LGAIQGFAGVSVRDGELHLNPALPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSL
RLNGQLITVAEESVFCLGDFILPFNGTATKHQEDE