Protein Info for ECD_01102 in Escherichia coli BL21

Annotation: thiamine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01636: APH
  • amino acids 25 to 233 (209 residues), 53 bits, E=4.5e-18
• TIGR02721: thiamine kinase
  • amino acids 26 to 270 (245 residues), 335.6 bits, E=7.6e-105
• PF01633: Choline_kinase
  • amino acids 162 to 208 (47 residues), 24.1 bits, 2.5e-09

Best Hits

Swiss-Prot: 100% identical to THIK_ECOHS: Thiamine kinase (thiK) from Escherichia coli O9:H4 (strain HS)

KEGG orthology group: K07251, thiamine kinase [EC: 2.7.1.89] (inferred from 99% identity to eco:b1106)

MetaCyc: 99% identical to thiamine kinase (Escherichia coli K-12 substr. MG1655)
Thiamine kinase. [EC: 2.7.1.89]

Predicted SEED Role

"Thiamine kinase (EC 2.7.1.89) @ Adenosylcobinamide kinase (EC 2.7.1.156)" (EC 2.7.1.156, EC 2.7.1.89)

MetaCyc Pathways

• superpathway of adenosylcobalamin salvage from cobinamide I (8/8 steps found)
• adenosylcobinamide-GDP salvage from cobinamide I (5/5 steps found)
• thiamine diphosphate salvage I (2/2 steps found)

KEGG Metabolic Maps

• Porphyrin and chlorophyll metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.156

Use Curated BLAST to search for 2.7.1.156 or 2.7.1.89

Protein Sequence (274 amino acids)

>ECD_01102 thiamine kinase (Escherichia coli BL21)
MPFRSNNPITRDELLSRFFPQFHHVTTFNSGLSGGSFLIEHQGQRFVVRQPHDPDAPQSA
FLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGAVKTYLPDTNELAGLLYYLHQQPRFG
WRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRPLRLSPLHMDVHAGNLVHSAS
GLKLIDWEYAGDGDIALELAAVWVENTEQHLQLVNDYATRAKIYPAQLWRQVRRWFPWLL
MLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ