Protein Info for ECD_00984 in Escherichia coli BL21

Annotation: tyrosine-protein kinase, role in O-antigen capsule formation

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 726 transmembrane" amino acids 33 to 51 (19 residues), see Phobius details amino acids 425 to 448 (24 residues), see Phobius details PF02706: Wzz" amino acids 16 to 107 (92 residues), 91.2 bits, see alignment E=1.1e-29 PF13807: GNVR" amino acids 368 to 448 (81 residues), 112 bits, see alignment E=2.5e-36 TIGR01007: capsular exopolysaccharide family" amino acids 513 to 719 (207 residues), 162.5 bits, see alignment E=4.7e-52 PF13614: AAA_31" amino acids 543 to 654 (112 residues), 39.1 bits, see alignment E=2e-13

Best Hits

Swiss-Prot: 100% identical to ETK_ECO57: Tyrosine-protein kinase etk (etk) from Escherichia coli O157:H7

KEGG orthology group: K00903, protein-tyrosine kinase [EC: 2.7.10.-] (inferred from 100% identity to eco:b0981)

MetaCyc: 100% identical to protein-tyrosine kinase Etk (Escherichia coli K-12 substr. MG1655)
Receptor protein-tyrosine kinase. [EC: 2.7.10.1, 2.7.10.2, 2.7.10.3, 2.7.12.1]

Predicted SEED Role

"Tyrosine-protein kinase Wzc (EC 2.7.10.2)" in subsystem Colanic acid biosynthesis (EC 2.7.10.2)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.10.-, 2.7.10.2

Use Curated BLAST to search for 2.7.10.- or 2.7.10.1 or 2.7.10.2 or 2.7.10.3 or 2.7.12.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (726 amino acids)

>ECD_00984 tyrosine-protein kinase, role in O-antigen capsule formation (Escherichia coli BL21)
MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLIAVAYSLLSTPIYQADT
LVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGKTIAELNLRDMVEQKYFPI
VGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGENGHYTLEGEEFTVNGMVGQRL
EKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETFTVSERSKESGMLELTMTGDDPQ
LITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQLPEVRSELDQAEEKLNVYRQQRDSV
DLNLEAKAVLEQIVNVDNQLNELTFREAEISQLYKKDHPTYRALLEKRQTLEQERKRLNK
RVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNRQQELSISKSSAIGNVRIIDPAVTQPQPV
KPKKALNVVLGFILGLFISVGAVLARAMLRRGVEAPEQLEEHGISVYATIPMSEWLDKRT
RLRKKNLFSNQQRHRTKNIPFLAVDNPADSAVEAVRALRTSLHFAMMETENNILMITGAT
PDSGKTFVSSTLAAVIAQSDQKVLFIDADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKV
IQHFGKGGFDVITRGQVPPNPSELLMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVV
GRSVGTSLLVARFGLNTAKEVSLSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGY
SYSEKE