Protein Info for ECD_00979 in Escherichia coli BL21

Annotation: putative HyaA chaperone

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 132 PF07449: HyaE" amino acids 10 to 116 (107 residues), 157 bits, see alignment E=7.7e-51

Best Hits

Swiss-Prot: 100% identical to HYAE_ECOLI: Hydrogenase-1 operon protein HyaE (hyaE) from Escherichia coli (strain K12)

KEGG orthology group: K03619, hydrogenase-1 operon protein HyaE (inferred from 100% identity to eco:b0976)

Predicted SEED Role

"Hydrogenase maturation factor hoxO" in subsystem Membrane-bound Ni, Fe-hydrogenase

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (132 amino acids)

>ECD_00979 putative HyaA chaperone (Escherichia coli BL21)
MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSDPKRTPEVSDNPVMIGE
LLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGNYRGVLNGIHPWAELINLM
RGLVEPQQERAS