Protein Info for ECD_00952 in Escherichia coli BL21

Annotation: 23S rRNA m(2)G2445 and m(7)G2069 methyltransferases, SAM-dependent

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 702 transmembrane" amino acids 459 to 479 (21 residues), see Phobius details PF22020: RlmL_1st" amino acids 4 to 58 (55 residues), 67.4 bits, see alignment 4.3e-22 PF02926: THUMP" amino acids 71 to 153 (83 residues), 59 bits, see alignment E=2.6e-19 PF01170: UPF0020" amino acids 162 to 375 (214 residues), 215.5 bits, see alignment E=3.1e-67 PF10672: Methyltrans_SAM" amino acids 475 to 659 (185 residues), 70.8 bits, see alignment E=5.9e-23 PF03602: Cons_hypoth95" amino acids 532 to 639 (108 residues), 37.7 bits, see alignment E=8.8e-13

Best Hits

Swiss-Prot: 100% identical to RLMKL_ECODH: Ribosomal RNA large subunit methyltransferase K/L (rlmL) from Escherichia coli (strain K12 / DH10B)

KEGG orthology group: K12297, ribosomal RNA large subunit methyltransferase L [EC: 2.1.1.173] (inferred from 100% identity to ebd:ECBD_2647)

MetaCyc: 100% identical to fused 23S rRNA m2G2445 methyltransferase and 23S rRNA m7G2069 methyltransferase (Escherichia coli K-12 substr. MG1655)
RXN0-6950 [EC: 2.1.1.264]; RXN-11574 [EC: 2.1.1.264, 2.1.1.173]

Predicted SEED Role

"23S rRNA (guanine-N-2-) -methyltransferase rlmL EC 2.1.1.-)"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.173 or 2.1.1.264

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (702 amino acids)

>ECD_00952 23S rRNA m(2)G2445 and m(7)G2069 methyltransferases, SAM-dependent (Escherichia coli BL21)
MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLVYQSLMWSRLASRIMLP
LGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTIRNSQYGAMKVKDAIVDAF
TRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLHLRGYRDRAGIAPIKETLAAA
IVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGLHRGRWGFSGWAQHDEAIWQEVK
AEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNARLAGIGELITFEVKDVAQLTNPLPK
GPYGTVLSNPPYGERLDSEPALIALHSLLGRIMKNQFGGWNLSLFSASPDLLSCLQLRAD
KQYKAKNGPLDCVQKNYHVAESTPDSKPAMVAEDYTNRLRKNLKKFEKWARQEGIECYRL
YDADLPEYNVAVDRYADWVVVQEYAPPKTLDAHKARQRLFDIIAATISVLGIAPNKLVLK
TRERQKGKNQYQKLGEKGEFLEVTEYNAHLWVNLTDYLDTGLFLDHRIARRMLGQMSKGK
DFLNLFSYTGSATVHAGLGGARSTTTVDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLA
WLREANEQFDLIFIDPPTFSNSKRMEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRG
FRMDLDGLAKLGLKAQEITQKTLSQDFARNRQIHNCWLITAA