Protein Info for ECD_00560 in Escherichia coli BL21
Annotation: isochorismate synthase 1
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 99% identical to ENTC_ECO57: Isochorismate synthase EntC (entC) from Escherichia coli O157:H7
KEGG orthology group: K02361, isochorismate synthase [EC: 5.4.4.2] (inferred from 100% identity to ebr:ECB_00560)MetaCyc: 99% identical to isochorismate synthase EntC (Escherichia coli K-12 substr. MG1655)
Isochorismate synthase. [EC: 5.4.4.2]
Predicted SEED Role
"Isochorismate synthase (EC 5.4.4.2) [enterobactin] siderophore" in subsystem Siderophore Enterobactin (EC 5.4.4.2)
MetaCyc Pathways
- superpathway of chorismate metabolism (54/59 steps found)
- superpathway of menaquinol-8 biosynthesis I (10/10 steps found)
- superpathway of demethylmenaquinol-8 biosynthesis I (9/9 steps found)
- 2-carboxy-1,4-naphthoquinol biosynthesis (7/7 steps found)
- superpathway of menaquinol-10 biosynthesis (9/10 steps found)
- superpathway of menaquinol-11 biosynthesis (9/10 steps found)
- superpathway of menaquinol-12 biosynthesis (9/10 steps found)
- superpathway of menaquinol-13 biosynthesis (9/10 steps found)
- superpathway of menaquinol-6 biosynthesis (9/10 steps found)
- superpathway of menaquinol-7 biosynthesis (9/10 steps found)
- superpathway of menaquinol-9 biosynthesis (9/10 steps found)
- superpathway of demethylmenaquinol-6 biosynthesis I (8/9 steps found)
- superpathway of demethylmenaquinol-9 biosynthesis (8/9 steps found)
- 2,3-dihydroxybenzoate biosynthesis (3/3 steps found)
- salicylate biosynthesis II (2/3 steps found)
- salicylate biosynthesis I (1/2 steps found)
- enterobactin biosynthesis (7/11 steps found)
- vibriobactin biosynthesis (4/9 steps found)
- bacillibactin biosynthesis (6/12 steps found)
- superpathway of phylloquinol biosynthesis (7/15 steps found)
KEGG Metabolic Maps
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of siderophore group nonribosomal peptides
- Ubiquinone and menaquinone biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 5.4.4.2
Use Curated BLAST to search for 5.4.4.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (391 amino acids)
>ECD_00560 isochorismate synthase 1 (Escherichia coli BL21) MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAVNGDSPDSPFQQKLAAL FADAKAQSIKNPVIVGAIPFDPRQPSSLYIPESWQSFSRQEKQTSARRFTRSQSLNVVER QAIPEQTTFEQMVARAAELTATPQVDKVVLSRLIDITTDAAIDSGVLLERLIAQNPVSYN FHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARRQPDEVLDREAGNRLLASEKDRH EHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWHLATPFEGKANSQENALTLACLLHP TPALSGFPHQAATQVIAELEPFDRELFGGIVGWCDSEGNGEWVVTIRCAKLRENQVRLFA GAGIVPASSPLGEWRETGVKLSTMLNVFGLH