Protein Info for ECD_00508 in Escherichia coli BL21

Annotation: DLP12 prophage; putative murein endopeptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 153 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details PF03245: Phage_lysis" amino acids 29 to 153 (125 residues), 182.3 bits, see alignment E=2e-58

Best Hits

Swiss-Prot: 98% identical to SPAN1_LAMBD: Spanin, inner membrane subunit (Rz) from Escherichia phage lambda

KEGG orthology group: K14744, putative Rz endopeptidase from lambdoid prophage DLP12 [EC: 3.4.-.-] (inferred from 100% identity to ebr:ECB_00508)

Predicted SEED Role

No annotation

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.-.-

Use Curated BLAST to search for 3.4.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (153 amino acids)

>ECD_00508 DLP12 prophage; putative murein endopeptidase (Escherichia coli BL21)
MNRVTAIISALVICIIVCLSWAVNHYRDNAITYKAQRDKNARELKLANAAITDMQMRQRD
VAALDEKYTKELANAKAENDALRDDVAAGRRRLHIKAVCQSVREATTASGVDNAASPRLA
DTAERDYFTLRERLITMQKQLEGTQKYINEQCR