Protein Info for ECD_00448 in Escherichia coli BL21

Annotation: Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1426 PF20148: DUF6531" amino acids 46 to 123 (78 residues), 66.1 bits, see alignment 5.8e-22 PF05593: RHS_repeat" amino acids 413 to 444 (32 residues), 30.4 bits, see alignment (E = 8.3e-11) amino acids 454 to 486 (33 residues), 25.1 bits, see alignment (E = 3.6e-09) amino acids 493 to 532 (40 residues), 24.5 bits, see alignment (E = 5.9e-09) amino acids 538 to 572 (35 residues), 33.5 bits, see alignment (E = 8.7e-12) amino acids 579 to 615 (37 residues), 44.9 bits, see alignment (E = 2.2e-15) amino acids 621 to 656 (36 residues), 25.3 bits, see alignment (E = 3.3e-09) amino acids 641 to 678 (38 residues), 32.4 bits, see alignment (E = 1.9e-11) TIGR01643: YD repeat (two copies)" amino acids 413 to 448 (36 residues), 28.3 bits, see alignment (E = 1.8e-10) amino acids 493 to 518 (26 residues), 19.7 bits, see alignment (E = 9.5e-08) amino acids 540 to 572 (33 residues), 21.3 bits, see alignment (E = 2.9e-08) amino acids 579 to 619 (41 residues), 43.3 bits, see alignment (E = 3.3e-15) amino acids 600 to 631 (32 residues), 24.7 bits, see alignment (E = 2.4e-09) amino acids 641 to 681 (41 residues), 21.2 bits, see alignment (E = 3.2e-08) PF03527: RHS" amino acids 1155 to 1190 (36 residues), 56.9 bits, see alignment (E = 2.9e-19) TIGR03696: RHS repeat-associated core domain" amino acids 1181 to 1258 (78 residues), 82 bits, see alignment 3.5e-27 PF14220: DUF4329" amino acids 1292 to 1411 (120 residues), 124.1 bits, see alignment E=5.7e-40

Best Hits

Swiss-Prot: 100% identical to RHSD_ECOLI: Protein RhsD (rhsD) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to ebd:ECBD_3159)

Predicted SEED Role

"core protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1426 amino acids)

>ECD_00448 Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor (Escherichia coli BL21)
MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMTSGNPVNPLLGAKVLPG
ETDLALPGPLPFILSRTYSSYRTRTPAPVGVFGPGWKAPSDIRLQLRDDALVLNDNGGRS
IHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLARLWGALPPDIRLSPHLYLATNSAQ
GPWWILGWSERVPGAEDVLPAPLPPYRVLTGLADRFGRTLTYRREAAGDLAGEITGVTDG
AGREFRLVLTTQAQRAEEARTSSLSSSDSSRPLSASAFPDTLPGTEYGPDRGIRLSAVWL
MHDPAYPESLPAAPLVRYTYTEAGELLAVYDRSNTQVRAFTYDAQHPGRMVAHRYAGRPE
MRYRYDDTGRVVEQLNPAGLSYRYLYEQDRITVTDSLNRREVLHTEGGAGLKRVVKKELA
DGSVTRSGYDAAGRLTAQTDAAGRRTEYGLNVVSGDITDITTPDGRETKFYYNDGNQLTA
VVYPDGLESRREYDEPGRLVSETSRSGETVRYRYDDAHSELPATTTDATGSTRQMTWSRY
GQLLAFTDCSGYQTRYEYDRFGQMTAVHREEGISLYRHYDNRGRLTSVKDAQGRETRYEY
NAAGDLTAVITPDGNRSETQYDAWGKAVSTTQGGLTRSMEYDAAGRVISLTNENGSHSVF
SYDALDRLVQQGGFDGRTQRYHYDLTGKLTQSEDEGLVILWYYDASDRITHRTVNGEPAE
QWQYDGHGWLTDISHLSEGHRVAVHYGYDDKGRLTGECQTVENPETGELLWQHETKHAYN
EQGLANRVTPDSLPPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETVRSFGSMAGSNA
AYELTSTYTPAGQLQSQHLNSLVYDRDYGWSDNGDLVRISGPRQTREYGYSATGRLESVR
TLAPDLDIRIPYATDPAGNRLPDPELHPDSTLTVWPDNRIAEDAHYVYRHDEYGRLTEKT
DRIPAGVIRTDDERTHHYHYDSQHRLVFYTRIQHGEPLVESRYLYDPLGRRMAKRVWRRE
RDLTGWMSLSRKPEVTWYGWDGDRLTTVQTDTTRIQTVYEPGSFTPLIRVETENGEREKA
QRRSLAETLQQEGSENGHGVVFPAELVRLLDRLEEEIRADRVSSESRAWLAQCGLTVEQL
ARQVEPEYTPARKAHLYHCDHRGLPLALISEDGNTAWSAEYDEWGNQLNEENPHHVYQPY
RLPGQQHDEESGLYYNRHRYYDPLQGRYITQDPMGLKGGWNLYQYPLNPLQQIDPMGLLQ
TWDDARSGACTGGVCGVLSRIIGPSKFDSTADAALDALKETQNRSLCNDMEYSGIVCKDT
NGKYFASKAETDNLRKESYPLKRKCPTGTDRVAAYHTHGADSHGDYVDEFFSSSDKNLVR
SKDNNLEAFYLATPDGRFEALNNKGEYIFIRNSVPGLSSVCIPYHD