Protein Info for ECD_00307 in Escherichia coli BL21

Annotation: 3-hydroxyphenylpropionic transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 403 signal peptide" amino acids 1 to 45 (45 residues), see Phobius details transmembrane" amino acids 54 to 74 (21 residues), see Phobius details amino acids 82 to 101 (20 residues), see Phobius details amino acids 107 to 128 (22 residues), see Phobius details amino acids 140 to 162 (23 residues), see Phobius details amino acids 169 to 189 (21 residues), see Phobius details amino acids 215 to 237 (23 residues), see Phobius details amino acids 252 to 273 (22 residues), see Phobius details amino acids 282 to 301 (20 residues), see Phobius details amino acids 307 to 328 (22 residues), see Phobius details amino acids 339 to 359 (21 residues), see Phobius details amino acids 370 to 390 (21 residues), see Phobius details PF07690: MFS_1" amino acids 21 to 303 (283 residues), 133.6 bits, see alignment E=1.2e-42 PF06779: MFS_4" amino acids 46 to 386 (341 residues), 30 bits, see alignment E=5.5e-11 PF00083: Sugar_tr" amino acids 48 to 199 (152 residues), 59.3 bits, see alignment E=5e-20

Best Hits

Swiss-Prot: 100% identical to MHPT_ECOLI: 3-(3-hydroxy-phenyl)propionate transporter (mhpT) from Escherichia coli (strain K12)

KEGG orthology group: K05819, MFS transporter, AAHS family, 3-hydroxyphenylpropionic acid transporter (inferred from 100% identity to eco:b0353)

MetaCyc: 100% identical to 3-hydroxyphenylpropionate/3-hydroxycinnamate:H+ symporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-61; TRANS-RXN0-457

Predicted SEED Role

"4-hydroxybenzoate transporter" in subsystem Cinnamic Acid Degradation or Gentisare degradation or Phenylpropanoid compound degradation or Salicylate and gentisate catabolism or p-Hydroxybenzoate degradation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (403 amino acids)

>ECD_00307 3-hydroxyphenylpropionic transporter (Escherichia coli BL21)
MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQAFALDKMQMGWIFSAGI
LGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWDFPSLVFARLMTGVGLGAA
LPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGFAGANLAWQTVFWVGGVVPLI
LVPLLMRWLPESAVFAGEKQSAPPLRALFAPETATATLLLWLCYFFTLLVVYMLINWLPL
LLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDKLRPVTMSLLIYSGMLASLLALGTV
SSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSSQIRATGVGTAVAVGRLGAMSGPLLAG
KMLALGTGTVGVMAASAPGILVAGLAVFILMSRRSRIQPCADA