Protein Info for ECD_00279 in Escherichia coli BL21

Annotation: putative metallo-dependent hydrolase domain deaminase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01979: Amidohydro_1
  • amino acids 113 to 378 (266 residues), 29.3 bits, E=5.5e-11
• PF07969: Amidohydro_3
  • amino acids 162 to 438 (277 residues), 52 bits, E=8.8e-18

Best Hits

Swiss-Prot: 100% identical to YAHJ_ECOLI: Uncharacterized protein YahJ (yahJ) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to eco:b0324)

Predicted SEED Role

"Cytosine deaminase (EC 3.5.4.1)" (EC 3.5.4.1)

MetaCyc Pathways

• pyrimidine nucleobases salvage II (2/2 steps found)
• pyrimidine ribonucleosides salvage III (2/2 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.5.4.1

Use Curated BLAST to search for 3.5.4.1

Protein Sequence (460 amino acids)

>ECD_00279 putative metallo-dependent hydrolase domain deaminase (Escherichia coli BL21)
MKESNSRREFLSQSGKMVTAAALFGTSVPLAHAAVAGTLNCEANNTMKITDPHYYLDNVL
LETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKLHPDATLPHYDAGGKLMLPTTRDM
HIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQPYTQERAEKLIDLLQSKGTTI
ARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFPQHGLLLSKSEPLMREAMQAGAH
YVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHLHETTPAGVAAINYMVETVEKTPQL
KGKLTISHAFALATLNEQQVDELANRMVVQQISIASTVPIGTLHMPLKQLHDKGVKVMTG
TDSVIDHWSPYGLGDMLEKANLYAQLYIRPNEQNLSRSLFLATGDVLPLNEKGERVWPKA
QDDASFVLVDASCSAEAVARISPRTATFHKGQLVWGSVAG