Protein Info for ECD_00268 in Escherichia coli BL21

Annotation: betaine aldehyde dehydrogenase, NAD-dependent

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01804: betaine-aldehyde dehydrogenase
  • amino acids 10 to 477 (468 residues), 821.9 bits, E=6.4e-252
• PF00171: Aldedh
  • amino acids 17 to 479 (463 residues), 601.2 bits, E=5.8e-185

Best Hits

Swiss-Prot: 99% identical to BETB_ECO55: NAD/NADP-dependent betaine aldehyde dehydrogenase (betB) from Escherichia coli (strain 55989 / EAEC)

KEGG orthology group: K00130, betaine-aldehyde dehydrogenase [EC: 1.2.1.8] (inferred from 100% identity to ebr:ECB_00268)

MetaCyc: 99% identical to betaine aldehyde dehydrogenase (Escherichia coli K-12 substr. MG1655)
Betaine-aldehyde dehydrogenase. [EC: 1.2.1.8]

Predicted SEED Role

"Betaine aldehyde dehydrogenase (EC 1.2.1.8)" in subsystem Choline and Betaine Uptake and Betaine Biosynthesis (EC 1.2.1.8)

MetaCyc Pathways

• choline-O-sulfate degradation (3/3 steps found)
• choline degradation I (2/2 steps found)
• glycine betaine biosynthesis I (Gram-negative bacteria) (2/2 steps found)
• glycine betaine biosynthesis II (Gram-positive bacteria) (2/2 steps found)
• glycine betaine biosynthesis III (plants) (2/3 steps found)
• β-alanine biosynthesis I (1/2 steps found)
• β-alanine biosynthesis IV (1/2 steps found)
• choline degradation IV (2/4 steps found)
• dimethylsulfoniopropanoate biosynthesis I (Wollastonia) (1/3 steps found)
• dimethylsulfoniopropanoate biosynthesis II (Spartina) (1/4 steps found)

KEGG Metabolic Maps

• Glycine, serine and threonine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.1.8

Protein Sequence (490 amino acids)

>ECD_00268 betaine aldehyde dehydrogenase, NAD-dependent (Escherichia coli BL21)
MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGREDVDRAVKSAQQGQKIW
AAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETSTVDIVTGADVLEYYAGLI
PALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIALWKSAPALAAGNAMIFKPSEV
TPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTEHPGIAKVSFTGGVASGKKVMAN
SAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMMANFFSSGQVCTNGTRVFVPAKCKA
AFEQKILARVERIRAGDVFDPQTNFGPLVSFPHRDNVLRYIVKGKEEGARVLCGGDVLKG
DDFDNGAWVAPTVFTDCSDDMTIVREEIFGPVMSILTYESEDEVIRRANDTDYGLAAGIV
TADLNRAHRVIHQLEAGICWINTWGESPAEMPVGGYKHSGIGRENGVMTLQSYTQVKSIQ
VEMAKFQSIF