Protein Info for ECD_00263 in Escherichia coli BL21

Annotation: ferridoxin-like LutB family protein; putative electron transport chain YkgEFG component

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00273: iron-sulfur cluster-binding protein
  • amino acids 18 to 453 (436 residues), 778.5 bits, E=8.7e-239
• PF02589: LUD_dom
  • amino acids 72 to 294 (223 residues), 177.5 bits, E=8.6e-56
• PF13183: Fer4_8
  • amino acids 309 to 376 (68 residues), 52.2 bits, E=2.5e-17
• PF13534: Fer4_17
  • amino acids 311 to 376 (66 residues), 31.2 bits, E=9e-11
• PF11870: LutB_C
  • amino acids 386 to 468 (83 residues), 41 bits, E=7.1e-14

Best Hits

Swiss-Prot: 100% identical to YKGF_ECOLI: Uncharacterized electron transport protein YkgF (ykgF) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to eco:b0307)

MetaCyc: 60% identical to component of an iron-sulfur oxidase linked to L-lactate utilization (Bacillus subtilis subtilis 168)
L-lactate dehydrogenase. [EC: 1.1.1.27]

Predicted SEED Role

"Predicted L-lactate dehydrogenase, Iron-sulfur cluster-binding subunit YkgF" in subsystem L-rhamnose utilization or Lactate utilization

MetaCyc Pathways

• hexitol fermentation to lactate, formate, ethanol and acetate (19/19 steps found)
• superpathway of glucose and xylose degradation (16/17 steps found)
• homolactic fermentation (12/12 steps found)
• superpathway of anaerobic sucrose degradation (17/19 steps found)
• Bifidobacterium shunt (13/15 steps found)
• heterolactic fermentation (15/18 steps found)
• pyruvate fermentation to acetate and (S)-lactate I (4/4 steps found)
• pyruvate fermentation to acetate and lactate II (4/4 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (10/13 steps found)
• pyruvate fermentation to (S)-lactate (1/1 steps found)

KEGG Metabolic Maps

• Cysteine metabolism
• Glycolysis / Gluconeogenesis
• Propanoate metabolism
• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.27

Protein Sequence (475 amino acids)

>ECD_00263 ferridoxin-like LutB family protein; putative electron transport chain YkgEFG component (Escherichia coli BL21)
MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDELGHWEEWRDRAAQIRD
HVLSNLDAYLYQLSEKVTQNGGHVYFARTKEDATRYILQVAQRKNARKVVKSKSMVTEEI
GVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHKDRHQIRRVLHERLGYEGPET
PEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCLVTNEGNARMCTTLPKTHIAVMG
MERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTGPREAGHVDGPEEFHLVIVDNGRSE
VLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYGSIYPGPIGAVISPLLGGYKDFKDLPY
ACSLCTACDNVCPVRIPLSKLILRHRRVMAEKGITAKAEQRAIKMFAYANSHPGLWKVGM
MAGAHAASWFINGGKTPLKFGAISDWMEARDLPEADGESFRSWFKKHQAQEKKNG