Protein Info for ECD_00256 in Escherichia coli BL21

Annotation: attaching and effacing protein, pathogenesis factor

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 410 transmembrane" amino acids 21 to 44 (24 residues), see Phobius details PF11924: IAT_beta" amino acids 76 to 320 (245 residues), 324.3 bits, see alignment E=4e-101

Best Hits

KEGG orthology group: K13735, adhesin/invasin (inferred from 100% identity to ebd:ECBD_3358)

Predicted SEED Role

"Putative adhesin"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (410 amino acids)

>ECD_00256 attaching and effacing protein, pathogenesis factor (Escherichia coli BL21)
MSHYKTGHKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMAARAQHAVQPRLSMGNT
TVTADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATAKANQEIQEWLGKYGTARV
KLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDDRTQSNIGFGWRHFSGNDWMA
GVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRASGWKKSPDVEDYQERPANGWDI
RAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQKDPHAISAEVTYTPVPLLTLSAGHK
QGKSGENDTRFGLEVNYRITLMAGVNPVGGSMWVDIEAPEGVTEKDYQFLPSKADHFSGG
KITRTFSTSKPGVYTFTFNALTYGGYEMTPVKVTINAVAAETENGEEEMP