Protein Info for ECD_00245 in Escherichia coli BL21

Annotation: PaoABC aldehyde oxidoreductase, 2Fe-2S subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00111: Fer2
  • amino acids 66 to 113 (48 residues), 45.3 bits, 1e-15
• PF01799: Fer2_2
  • amino acids 133 to 221 (89 residues), 101 bits, E=4.8e-33

Best Hits

Swiss-Prot: 99% identical to PAOA_ECOLI: Aldehyde oxidoreductase iron-sulfur-binding subunit PaoA (paoA) from Escherichia coli (strain K12)

KEGG orthology group: K13483, xanthine dehydrogenase YagT iron-sulfur-binding subunit (inferred from 99% identity to eco:b0286)

MetaCyc: 99% identical to aldehyde dehydrogenase, Fe-S subunit (Escherichia coli K-12 substr. MG1655)
Carboxylate reductase. [EC: 1.2.99.6]; 1.2.98.- [EC: 1.2.99.6]; 1.2.98.- [EC: 1.2.99.6]

Predicted SEED Role

"Periplasmic aromatic aldehyde oxidoreductase, iron-sulfur subunit YagT"

KEGG Metabolic Maps

• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.99.6

Protein Sequence (229 amino acids)

>ECD_00245 PaoABC aldehyde oxidoreductase, 2Fe-2S subunit (Escherichia coli BL21)
MSNQGEYPEDNRVGKHEPHDFSLTRRDLIKVSAATAATAVVYPHSTLAASVPAATPAPEI
MPLTLKVNGKTEQLEVDTRTTLLDALRENLHLIGTKKGCDHGQCGACTVLVNGRRLNACL
TLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGYCTSGQICSSVAVLKEIQDGI
PSHVTVDLVSAPETTADEIRERMSGNICRCGAYANILAAIEDAAGEIKS