Protein Info for ECD_00185 in Escherichia coli BL21

Annotation: putative lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 129 PF00903: Glyoxalase" amino acids 7 to 126 (120 residues), 103 bits, see alignment E=2.2e-33 PF13468: Glyoxalase_3" amino acids 8 to 106 (99 residues), 25.7 bits, see alignment E=1.7e-09 PF13669: Glyoxalase_4" amino acids 8 to 102 (95 residues), 47.9 bits, see alignment E=2.3e-16

Best Hits

Swiss-Prot: 98% identical to YAER_ECOLI: Uncharacterized protein YaeR (yaeR) from Escherichia coli (strain K12)

KEGG orthology group: K08234, glyoxylase I family protein (inferred from 98% identity to eco:b0187)

Predicted SEED Role

"FIG01280259: hypothetical protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (129 amino acids)

>ECD_00185 putative lyase (Escherichia coli BL21)
MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREVRDSWKGDLALNGQYVIELFS
FPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEAIRVDPYTQKRFTFFNDPD
GLPLELYEQ