Protein Info for ECD_00153 in Escherichia coli BL21

Annotation: glutamate-1-semialdehyde aminotransferase (aminomutase)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00713: glutamate-1-semialdehyde-2,1-aminomutase
  • amino acids 3 to 425 (423 residues), 685.1 bits, E=1.5e-210
• PF00202: Aminotran_3
  • amino acids 32 to 395 (364 residues), 270.3 bits, E=1.2e-84

Best Hits

Swiss-Prot: 100% identical to GSA_ECO8A: Glutamate-1-semialdehyde 2,1-aminomutase (hemL) from Escherichia coli O8 (strain IAI1)

KEGG orthology group: K01845, glutamate-1-semialdehyde 2,1-aminomutase [EC: 5.4.3.8] (inferred from 99% identity to eco:b0154)

MetaCyc: 99% identical to glutamate-1-semialdehyde 2,1-aminomutase (Escherichia coli K-12 substr. MG1655)
Glutamate-1-semialdehyde 2,1-aminomutase. [EC: 5.4.3.8]

Predicted SEED Role

"Glutamate-1-semialdehyde aminotransferase (EC 5.4.3.8)" in subsystem Experimental tye or Heme and Siroheme Biosynthesis (EC 5.4.3.8)

MetaCyc Pathways

• superpathway of heme b biosynthesis from glutamate (10/10 steps found)
• tetrapyrrole biosynthesis I (from glutamate) (6/6 steps found)
• adenosylcobalamin biosynthesis II (aerobic) (17/33 steps found)
• adenosylcobalamin biosynthesis I (anaerobic) (16/36 steps found)

KEGG Metabolic Maps

• Porphyrin and chlorophyll metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.4.3.8

Protein Sequence (426 amino acids)

>ECD_00153 glutamate-1-semialdehyde aminotransferase (aminomutase) (Escherichia coli BL21)
MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYLYDVDGKAYIDYVGSWG
PMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTELVPTMDMVRMVNSGTEATM
SAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTLGQPNSPGVPADFAKHTLTCT
YNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPEFLPGLRALCDEFGALLIIDEVM
TGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAFGGRRDVMDALAPTGPVYQAGTLSG
NPIAMAAGFACLNEVAQPGVHETLDELTSRLAEGLLEAAEEAGIPLVVNHVGGMFGIFFT
DAESVTCYQDVMACDVERFKRFFHMMLDEGVYLAPSAFEAGFMSVAHSMEDINNTIDAAR
RVFAKL