Protein Info for DZA65_RS22605 in Dickeya dianthicola ME23

Annotation: formate-dependent uric acid utilization protein AegA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF13247: Fer4_11
  • amino acids 53 to 140 (88 residues), 48.1 bits, E=8.1e-16
• PF13237: Fer4_10
  • amino acids 82 to 133 (52 residues), 25.6 bits, 6.8e-09
• PF12797: Fer4_2
  • amino acids 82 to 99 (18 residues), 24.6 bits, see alignment (E = 1.2e-08)
• PF00037: Fer4
  • amino acids 83 to 103 (21 residues), 32 bits, see alignment (E = 5.4e-11)
• PF12838: Fer4_7
  • amino acids 86 to 136 (51 residues), 27.4 bits, 2.6e-09
• TIGR01318: glutamate synthase, small subunit
  • amino acids 185 to 646 (462 residues), 790.9 bits, E=1.7e-242
• PF14691: Fer4_20
  • amino acids 199 to 309 (111 residues), 124.2 bits, E=1.6e-39
• PF07992: Pyr_redox_2
  • amino acids 323 to 635 (313 residues), 68.6 bits, E=4.6e-22
• PF00070: Pyr_redox
  • amino acids 323 to 394 (72 residues), 23.7 bits, E=3.9e-08
• PF01494: FAD_binding_3
  • amino acids 324 to 354 (31 residues), 27 bits, see alignment (E = 1.9e-09)
• PF13450: NAD_binding_8
  • amino acids 326 to 360 (35 residues), 34.6 bits, see alignment (E = 1.4e-11)

Best Hits

KEGG orthology group: None (inferred from 96% identity to ddd:Dda3937_01085)

Predicted SEED Role

"Glutamate synthase [NADPH] small chain (EC 1.4.1.13)" in subsystem Ammonia assimilation or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.1.13)

MetaCyc Pathways

• ammonia assimilation cycle III (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-glutamine degradation II (1/1 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (3/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (3/4 steps found)
• L-glutamate and L-glutamine biosynthesis (5/7 steps found)
• L-citrulline biosynthesis (5/8 steps found)
• superpathway of L-citrulline metabolism (7/12 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Glutamate metabolism
• Nitrogen metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.4.1.13

Use Curated BLAST to search for 1.4.1.13

Sequence Analysis Tools

See A0A385Y4N3 at UniProt or InterPro

Protein Sequence (667 amino acids)

>DZA65_RS22605 formate-dependent uric acid utilization protein AegA (Dickeya dianthicola ME23)
MNQFVIANAKDCIGCNACEIACVISHNHGRYPENRDDFHPRIRVFHQGEQHTAVTCRHCE
DSPCAKVCPTQALVRKQGGIQLVAEKCIGCKTCVLACPFGAISVEAQPQGAVAHKCDLCI
GRPQGQACVEACPTQALHFVSERSLEEQRRTKQLRMAQTAMPGTPRAVPTFAEIACRPLD
KRKNWPRQDAVKKPLEERVTTFGEIYHGFSAEQTLDQASRCLHCGQHAVCEWTCPLHNNI
PALLQLVQEGRILDAAELSHRTSSLPEICGRVCPQDRLCEGACTLGKGYGAVTVGNIERY
ITDTAMSMGWQPDLSQVKPTGKRAAIIGAGPAGLACADVLARHGVQAVVFDRHPEIGGLL
TFGIPAFKLDKSVLTRRREIFSAMGIEFRLNTEVGTDIGMAQLLAEFDTVFLGVGTYRSM
KAGLENEDAPGVYDALPFLIANTKQVMGLPTLPDEPYVSMQGKRVLVLGGGDTAMDCLRT
SIRQGATSVTCAYRRDEANMPGSRKEVKNSREEGVEFLFNVQPLKICLNDDGEVCGVSLI
RTEMGEPDASGRRRPKPVPGSEFVQPAEAVITAFGFQTHSMPWLQDARIALDSWGHITTT
QPGDRHPCQTSHSQVFAGGDAVRGADLVVTAIADGRKAAQGMLRVMGLESQDMSLPVNAV
VQQENRP