Protein Info for DZA65_RS21935 in Dickeya dianthicola ME23

Annotation: two-component system sensor histidine kinase DcuS

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 537 transmembrane" amino acids 12 to 35 (24 residues), see Phobius details amino acids 153 to 169 (17 residues), see Phobius details amino acids 175 to 195 (21 residues), see Phobius details PF17203: sCache_3_2" amino acids 44 to 171 (128 residues), 61.8 bits, see alignment E=2.3e-20 PF00989: PAS" amino acids 216 to 316 (101 residues), 26.9 bits, see alignment E=1.2e-09 PF14689: SPOB_a" amino acids 335 to 375 (41 residues), 32.7 bits, see alignment 1.4e-11 PF02518: HATPase_c" amino acids 426 to 530 (105 residues), 77 bits, see alignment E=4.8e-25 PF14501: HATPase_c_5" amino acids 427 to 521 (95 residues), 35.2 bits, see alignment E=3e-12

Best Hits

Swiss-Prot: 56% identical to DCUS_ECOLI: Sensor histidine kinase DcuS (dcuS) from Escherichia coli (strain K12)

KEGG orthology group: K07701, two-component system, CitB family, sensor histidine kinase DcuS [EC: 2.7.13.3] (inferred from 97% identity to dze:Dd1591_4102)

MetaCyc: 56% identical to sensor histidine kinase DcuS (Escherichia coli K-12 substr. MG1655)
Histidine kinase. [EC: 2.7.13.3]

Predicted SEED Role

"Fumarate respiration sensor kinase protein DcuS"

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A3A4CFE0 at UniProt or InterPro

Protein Sequence (537 amino acids)

>DZA65_RS21935 two-component system sensor histidine kinase DcuS (Dickeya dianthicola ME23)
MSKKKTPLKLGTSIILMVAAVIGSVLLVVYALLFFRITELTETHLKDKAFAIARTVANSP
LVVDDLKGIGDPMRVQRFSESVKSRNHLLFVIVTDMDGIRHSHPEPGQIGRHFIGDDLYP
ALLGLENTAVNRGVLDPALRVFTPVFDDNNRQLGVVVVGISLSSVQSVIDENRWIIPWTI
LFGALVGLLGTYFLVKTLKHIMLGFEPFEISNLFEQRNAMLKQIKEGVIAVDTDLRVTIV
NDEAKRLFSQHGSGETLAIGSTISRWPALMNLEKVLESGSPRQDEEINFNGNLLLINTVP
VVVKGDIIGAIATFRDKTEVSQLLQRLTGMSYYADALRAQSHEFMNKLHVILGMLHLKYY
PQLEEYILKTANNYQAEIGSIIRKVKSPVIAGFLLGKINRARDLGVTLSISEDSLLPDTD
DSQATNELITVLGNLIENAMDALSGLENREISVTFHHQDGQLHCTVSDDGPGIAPDIQQR
IYREGFSTKGSGRGIGLYLTRQSLEKIGGTIDFESEPDVYTQFFVNIPYQARQFDHD