Protein Info for DZA65_RS21485 in Dickeya dianthicola ME23

Annotation: primosomal protein N'

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 732 PF17764: PriA_3primeBD" amino acids 5 to 101 (97 residues), 98.7 bits, see alignment E=5e-32 PF21213: PriA-like_WH" amino acids 114 to 176 (63 residues), 110.5 bits, see alignment E=1.1e-35 PF04851: ResIII" amino acids 198 to 334 (137 residues), 51.1 bits, see alignment E=5.4e-17 PF00270: DEAD" amino acids 202 to 364 (163 residues), 70.2 bits, see alignment E=6.4e-23 TIGR00595: primosomal protein N'" amino acids 221 to 729 (509 residues), 673.9 bits, see alignment E=6.9e-207 PF18319: PriA_CRR" amino acids 445 to 471 (27 residues), 31.4 bits, see alignment (E = 5e-11) PF00271: Helicase_C" amino acids 504 to 589 (86 residues), 32.7 bits, see alignment E=2.7e-11 PF18074: PriA_C" amino acids 632 to 729 (98 residues), 71.4 bits, see alignment E=3.3e-23

Best Hits

Swiss-Prot: 73% identical to PRIA_ECOLI: Primosomal protein N' (priA) from Escherichia coli (strain K12)

KEGG orthology group: K04066, primosomal protein N' (replication factor Y) (superfamily II helicase) [EC: 3.6.4.-] (inferred from 98% identity to ddd:Dda3937_03886)

MetaCyc: 73% identical to primosomal protein N' (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"Helicase PriA essential for oriC/DnaA-independent DNA replication" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase or DNA-replication

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.-

Use Curated BLAST to search for 3.6.4.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A385Y2W9 at UniProt or InterPro

Protein Sequence (732 amino acids)

>DZA65_RS21485 primosomal protein N' (Dickeya dianthicola ME23)
MPVVQVALPVPLTRTFDYRLSQGVTPPVPGVRVSVPFGNRKMIGIVTALNAGSGLPLEQL
KPLHDVLDDATLFPDSLWRILLWAAEYYHYPLGEVLFHALPILLRQGKPAHSAPLWQWFA
TEQGRATSLNALKRAPKQQQALAALLQGPLYRHQVAETELTEAALQALREKGLSELRALP
QQVKDWRDDFSLTGERLRLNTEQATAVGAIRSEDDHFAAWLLAGITGSGKTEVYLSVLEN
ILAQGKQALVLVPEIGLTPQTIARFRDRFNAPVEALHSGLNDGERLSVWLRARQGEAAIV
IGTRSALFTPFARLGLIVIDEEHDGSYKQQEGWRYHARDLAVFRARQEDIPIVMGSATPA
LETLYNVQIGKYRQLRLSKRAGNARLAQQHLLDLKGLPLTTGLSQPLINRIRHHLAADNQ
VILFLNRRGFAPVVLCHECGWIAECQRCDSYYTLHQHQRMMRCHHCDSQRPVPQQCPQCG
STHLVPVGLGTEQLEQALPTLFPEAPITRIDRDTTSRKGALEQQLAQVRQGGARILIGTQ
MLAKGHHFPDVTLVALLDVDSSLFSADFRATERFAQLYTQVSGRAGRAGKAGEVVLQTHH
PEHPLLQTLLHQGYDAFASQTLKERQSVFLPPFTSHVLFRADDHDNQQASLFLQQLRNLL
EASPLRDDSLWLMGPVPALQPKRAGRFRWQLLLQHPSRATLQRLIRNSMPLIDTLPQTRK
VKWVMDVDPTDN