Protein Info for DZA65_RS21310 in Dickeya dianthicola ME23

Annotation: ketol-acid reductoisomerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF07991: IlvN
  • amino acids 35 to 204 (170 residues), 189 bits, E=4.9e-60
• TIGR00465: ketol-acid reductoisomerase
  • amino acids 35 to 368 (334 residues), 442.7 bits, E=3e-137
• PF01450: IlvC
  • amino acids 210 to 342 (133 residues), 80.8 bits, E=1.3e-26
  • amino acids 353 to 482 (130 residues), 59.2 bits, E=5.8e-20

Best Hits

Swiss-Prot: 96% identical to ILVC_PECCP: Ketol-acid reductoisomerase (NADP(+)) (ilvC) from Pectobacterium carotovorum subsp. carotovorum (strain PC1)

KEGG orthology group: K00053, ketol-acid reductoisomerase [EC: 1.1.1.86] (inferred from 98% identity to ddd:Dda3937_00256)

MetaCyc: 92% identical to ketol-acid reductoisomerase (NADP+) (Escherichia coli K-12 substr. MG1655)
Ketol-acid reductoisomerase. [EC: 1.1.1.86]; 1.1.1.86 [EC: 1.1.1.86]; 2-dehydropantoate 2-reductase. [EC: 1.1.1.86, 1.1.1.169]

Predicted SEED Role

"Ketol-acid reductoisomerase (EC 1.1.1.86)" in subsystem Branched-Chain Amino Acid Biosynthesis or Coenzyme A Biosynthesis (EC 1.1.1.86)

MetaCyc Pathways

• superpathway of branched chain amino acid biosynthesis (17/17 steps found)
• superpathway of L-isoleucine biosynthesis I (13/13 steps found)
• superpathway of coenzyme A biosynthesis I (bacteria) (9/9 steps found)
• L-isoleucine biosynthesis I (from threonine) (7/7 steps found)
• L-valine biosynthesis (4/4 steps found)
• phosphopantothenate biosynthesis I (4/4 steps found)
• pyruvate fermentation to isobutanol (engineered) (4/5 steps found)
• phosphopantothenate biosynthesis III (archaea) (2/4 steps found)
• superpathway of L-threonine metabolism (12/18 steps found)
• L-isoleucine biosynthesis III (4/7 steps found)
• superpathway of coenzyme A biosynthesis II (plants) (5/10 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Pantothenate and CoA biosynthesis
• Valine, leucine and isoleucine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.169

Use Curated BLAST to search for 1.1.1.169 or 1.1.1.86

Sequence Analysis Tools

See A0A3A4C844 at UniProt or InterPro

Protein Sequence (492 amino acids)

>DZA65_RS21310 ketol-acid reductoisomerase (Dickeya dianthicola ME23)
MANYFNTLNLRQQLAQLGKCRFMGRDEFADEASYLKGKKVVIVGCGAQGLNQGLNMRDSG
LDIAYALRAEAIAEKRASWRKATENGFKVGTYEELIPQADLVVNLTPDKQHSAVVQAVQP
LMKQGAALGYSHGFNIVEVGEQVRKDITVVMVAPKCPGTEVREEYKRGFGVPTLIAVHPE
NDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDLMGEQTILCGMLQAGSLLSFDKL
VAEGVDPAYAEKLIQFGWETITEALKQGGITLMMDRLSNPAKLRAFALSEQLKGIMAPLF
QKHMDDIISGAFSSGMMADWANDDVKLLTWREETGKTAFENAPQFDGKITEQEYFDNGVL
MIAMVKAGVELAFETMVSSGIIEESAYYESLHELPLIANTIARKRLYEMNVVISDTAEYG
NYLFANAAVPLLKGAFMDSLQPGDLGKPVAATSVDNAQLRDVNEAVRNHPIEVIGKKLRG
YMTDMKRIAVAG