Protein Info for DZA65_RS21030 in Dickeya dianthicola ME23

Annotation: lysophospholipase L2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 330 PF12146: Hydrolase_4" amino acids 53 to 312 (260 residues), 193.6 bits, see alignment E=6.8e-61 PF00561: Abhydrolase_1" amino acids 56 to 311 (256 residues), 96.1 bits, see alignment E=5.5e-31 PF12697: Abhydrolase_6" amino acids 57 to 308 (252 residues), 44.1 bits, see alignment E=8.2e-15

Best Hits

Swiss-Prot: 61% identical to PLDB_ECOL6: Lysophospholipase L2 (pldB) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K01048, lysophospholipase [EC: 3.1.1.5] (inferred from 94% identity to dze:Dd1591_0196)

MetaCyc: 61% identical to lysophospholipase L2 (Escherichia coli K-12 substr. MG1655)
Lysophospholipase. [EC: 3.1.1.5]; 3.1.1.5 [EC: 3.1.1.5]; RXN0-7122 [EC: 3.1.1.5]

Predicted SEED Role

"Lysophospholipase L2 (EC 3.1.1.5)" in subsystem Synechocystis experimental or Triacylglycerol metabolism (EC 3.1.1.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.1.5

Use Curated BLAST to search for 3.1.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A3A4CP40 at UniProt or InterPro

Protein Sequence (330 amino acids)

>DZA65_RS21030 lysophospholipase L2 (Dickeya dianthicola ME23)
MNPYPECLLTREWQYAAFVTGPLLDFWRQRQEGMFIGVDDVPIRFVRFTSPSHQHLVVVL
PGRTDSYVKYAEAAYDLFQCGYDVLMMDHRGQGRSDRLLKDRHRGHVKRFGDYVDDVATL
WQQQVASERYTKRFALAHSMGGAVLAQFLARQPQAFDAAALCAPMCGILLPMPRWLAWRI
LDWAERYPTIRDYYAIGTSQWRPLPFMVNVLTHSHARYRRHVRFYADDPNLRIGGPTYHW
VREALLVETQLLQQAPAITTPLLLLQAEEDRVVDNRCQDAFCQALAAAGHPCADGRPYVI
RGARHDILSEKDAMRAEAFGRILQHFNDYD