Protein Info for DZA65_RS20865 in Dickeya dianthicola ME23

Annotation: pimeloyl-ACP methyl ester esterase BioH

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 255 TIGR01738: pimelyl-[acyl-carrier protein] methyl ester esterase" amino acids 10 to 252 (243 residues), 320.8 bits, see alignment E=3.2e-100 PF12697: Abhydrolase_6" amino acids 16 to 247 (232 residues), 80.6 bits, see alignment E=4e-26 PF12146: Hydrolase_4" amino acids 16 to 237 (222 residues), 42.9 bits, see alignment E=5.8e-15 PF00561: Abhydrolase_1" amino acids 16 to 242 (227 residues), 109.2 bits, see alignment E=4.1e-35

Best Hits

Swiss-Prot: 75% identical to BIOH_PECCP: Pimeloyl-[acyl-carrier protein] methyl ester esterase (bioH) from Pectobacterium carotovorum subsp. carotovorum (strain PC1)

KEGG orthology group: K02170, biotin biosynthesis protein BioH (inferred from 94% identity to dze:Dd1591_0236)

MetaCyc: 63% identical to pimeloyl-acyl carrier protein methyl ester esterase (Escherichia coli K-12 substr. MG1655)
RXN-11483 [EC: 3.1.1.85]; Carboxylesterase. [EC: 3.1.1.85, 3.1.1.1]

Predicted SEED Role

"Biotin synthesis protein BioH"

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.1.1 or 3.1.1.85

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A3A4CCW8 at UniProt or InterPro

Protein Sequence (255 amino acids)

>DZA65_RS20865 pimeloyl-ACP methyl ester esterase BioH (Dickeya dianthicola ME23)
MELLHWQTEGEGTYELVMLHGWGMNSQVWNSIVMRLAPHFRLHRVDLPGYGQSQGFGVMS
LADMAQQVLAQAPERAVWLGWSLGGLVASQAALSAPERVNALITMASSPCFEARDGWPGI
RPQVLSGFQQMLSEDFFGTIERFLALQTLGTENARKDARLLKVALSGQPAPSTEVLNAGL
GILRDTDLREPLSALTMPFLRLYGALDGLVPRKVAALMDERYPHSTSVVIPKAAHAPFIS
HPEVFVEQVVGFCAG